Proteomic analysis for the assessment of diabetic renal damage in humans
- 26 October 2004
- journal article
- Published by Portland Press Ltd. in Clinical Science
- Vol. 107 (5), 485-495
- https://doi.org/10.1042/cs20040103
Abstract
Renal disease in patients with Type II diabetes is the leading cause of terminal renal failure and a major healthcare problem. Hence early identification of patients prone to develop this complication is important. Diabetic renal damage should be reflected by a change in urinary polypeptide excretion at a very early stage. To analyse these changes, we used an online combination of CE/MS (capillary electrophoresis coupled with MS), allowing fast and accurate evaluation of up to 2000 polypeptides in urine. Employing this technology, we have examined urine samples from 39 healthy individuals and from 112 patients with Type II diabetes mellitus and different degrees of albumin excretion rate. We established a ‘normal’ polypeptide pattern in the urine of healthy subjects. In patients with Type II diabetes and normal albumin excretion rate, the polypeptide pattern in urine differed significantly from normal, indicating a specific ‘diabetic’ pattern of polypeptide excretion. In patients with higher grade albuminuria, we were able to detect a polypeptide pattern indicative of ‘diabetic renal damage’. We also found this pattern in 35% of those patients who had low-grade albuminuria and in 4% of patients with normal albumin excretion. Moreover, we could identify several of the indicative polypeptides using MS/MS sequencing. We conclude that proteomic analysis with CE/MS permits fast and accurate identification and differentiation of polypeptide patterns in urine. Longitudinal studies should explore the potential of this powerful diagnostic tool for early detection of diabetic renal damage.This publication has 27 references indexed in Scilit:
- Proteomic patterns established with capillary electrophoresis and mass spectrometry for diagnostic purposesKidney International, 2004
- Relaxin-1–deficient mice develop an age-related progression of renal fibrosisKidney International, 2004
- Relaxin‐like peptides in cancerInternational Journal of Cancer, 2003
- Detection and analysis of urinary peptides by on-line liquid chromatography and mass spectrometry: application to patients with renal Fanconi syndromeClinical Science, 2003
- Diabetic Nephropathy in Type 2 Diabetes Prevention and Patient ManagementJournal of the American Society of Nephrology, 2003
- Proteomic analysis of normal human urinary proteins isolated by acetone precipitation or ultracentrifugationKidney International, 2002
- Two‐dimensional electrophoresis of human urinary proteins following concentration by dye precipitationElectrophoresis, 1996
- Protein analysis on a genomic scaleJournal of Biotechnology, 1995
- Screening and management of microalbuminuria in patients with diabetes mellitus: recommendations to the scientific advisory board of the nationals Kidney Foundation from an Ad Hoc Committee of the council on diabetes mel of the national kidney foundationAmerican Journal of Kidney Diseases, 1995
- Two‐dimensional electrophoresis of proteins: An updated protocol and implications for a functional analysis of the genomeElectrophoresis, 1995