Virus-Induced Gene Silencing, a Post Transcriptional Gene Silencing Method
Open Access
- 15 June 2009
- journal article
- review article
- Published by Hindawi Limited in International Journal of Plant Genomics
- Vol. 2009, 1-8
- https://doi.org/10.1155/2009/198680
Abstract
Virus-induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA-mediated gene silencing. There are a number of viruses which have been modified to silence the gene of interest effectively with a sequence-specific manner. Therefore, different types of methodologies have been advanced and modified for VIGS approach. Virus-derived inoculations are performed on host plants using different methods such as agro-infiltration and in vitro transcriptions. VIGS has many advantages compared to other loss-of-gene function approaches. The approach provides the generation of rapid phenotype and no need for plant transformation. The cost of VIGS experiment is relatively low, and large-scale analysis of screening studies can be achieved by the VIGS. However, there are still limitations of VIGS to be overcome. Nowadays, many virus-derived vectors are optimized to silence more than one host plant such as TRV-derived viral vectors which are used for Arabidopsis and Nicothiana benthamiana. By development of viral silencing systems monocot plants can also be targeted as silencing host in addition to dicotyledonous plants. For instance, Barley stripe mosaic virus (BSMV)-mediated VIGS allows silencing of barley and wheat genes. Here we summarize current protocols and recent modified viral systems to lead silencing of genes in different host species.Keywords
This publication has 45 references indexed in Scilit:
- Virus-induced gene silencing in Medicago truncatula and Lathyrus odorataVirus Research, 2008
- Proteolytic Processing of Turnip Yellow Mosaic Virus Replication Proteins and Functional Impact on InfectivityJournal of Virology, 2007
- Virus-induced gene silencing as a tool for functional analyses in the emerging model plant Aquilegia (columbine, Ranunculaceae)Plant Methods, 2007
- Efficient Virus-Induced Gene Silencing in ArabidopsisPlant Physiology, 2006
- Agroinjection of Tomato Fruits. A Tool for Rapid Functional Analysis of Transgenes Directly in FruitPlant Physiology, 2006
- Potato Virus X-Induced Gene Silencing in Leaves and Tubers of PotatoPlant Physiology, 2004
- MicroRNAs: Genomics, Biogenesis, Mechanism, and FunctionCell, 2004
- Two MAPK cascades, NPR1, and TGA transcription factors play a role in Pto‐mediated disease resistance in tomatoThe Plant Journal, 2003
- Gene silencing from plant DNA carried by a GeminivirusThe Plant Journal, 1998
- Virus-induced gene silencing in infected and transgenic plantsTrends in Plant Science, 1997