Isolation, Structure Elucidation, and Biological Activity of Virgineone from Lachnum virgineum Using the Genome-Wide Candida albicans Fitness Test
- 30 December 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Natural Products
- Vol. 72 (1), 136-141
- https://doi.org/10.1021/np800511r
Abstract
A glycosylated tetramic acid, virgineone (1), was isolated from saprotrophic Lachnum virgineum. The antifungal activity of the fermentation extract of L. virgineum was characterized in the Candida albicans fitness test as distinguishable from other natural products tested. Bioassay-guided fractionation yielded 1, a tyrosine-derived tetramic acid with a C-22 oxygenated chain and a beta-mannose. It displayed broad-spectrum antifungal activity against Candida spp. and Aspergillus fumigatus with a MIC of 4 and 16 microg/mL, respectively. Virgineone was also identified in a number of Lachnum strains collected from diverse geographies and habitats.Keywords
This publication has 22 references indexed in Scilit:
- Isolation and Structure Elucidation of Parnafungins, Antifungal Natural Products that Inhibit mRNA PolyadenylationJournal of the American Chemical Society, 2008
- PAP Inhibitor with In Vivo Efficacy Identified by Candida albicans Genetic Profiling of Natural ProductsCell Chemical Biology, 2008
- Update on Azole AntifungalsSeminars in Respiratory and Critical Care Medicine, 2008
- Will resistance in fungi emerge on a scale similar to that seen in bacteria?European Journal of Clinical Microbiology & Infectious Diseases, 2008
- Mechanism-of-Action Determination of GMP Synthase Inhibitors and Target Validation in Candida albicans and Aspergillus fumigatusCell Chemical Biology, 2007
- Genome-Wide Fitness Test and Mechanism-of-Action Studies of Inhibitory Compounds in Candida albicansPLoS Pathogens, 2007
- Regulation of Cell-Surface Genes and Biofilm Formation by the C. albicans Transcription Factor Bcr1pCurrent Biology, 2005
- Novel Tetramic Acids and Pyridone Alkaloids, Militarinones B, C, and D, from the Insect Pathogenic Fungus Paecilomyces militarisJournal of Natural Products, 2003
- Update on antifungals targeted to the cell wall: focus on β-1,3-glucan synthase inhibitorsExpert Opinion on Investigational Drugs, 2001
- Amphotericin B: 30 Years of Clinical ExperienceClinical Infectious Diseases, 1990