Epithelial cell pro‐inflammatory cytokine response differs across dental plaque bacterial species
- 14 December 2009
- journal article
- Published by Wiley in Journal of Clinical Periodontology
- Vol. 37 (1), 24-29
- https://doi.org/10.1111/j.1600-051x.2009.01505.x
Abstract
Aim: The dental plaque is comprised of numerous bacterial species, which may or may not be pathogenic. Human gingival epithelial cells (HGECs) respond to perturbation by various bacteria of the dental plaque by production of different levels of inflammatory cytokines, which is a putative reflection of their virulence. The aim of the current study was to determine responses in terms of interleukin (IL)‐1β, IL‐6, IL‐8 and IL‐10 secretion induced by Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and Streptococcus gordonii in order to gauge their virulence potential. Materials and Methods: HGECs were challenged with the four bacterial species, live or heat killed, at various multiplicity of infections and the elicited IL‐1β, IL‐6, IL‐8 and IL‐10 responses were assayed by enzyme‐linked immunosorbent assay. Results: Primary HGECs challenged with live P. gingivalis produced high levels of IL‐1β, while challenge with live A. actinomycetemcomitans gave high levels of IL‐8. The opportunistic pathogen F. nucleatum induces the highest levels of pro‐inflammatory cytokines, while the commensal S. gordonii is the least stimulatory. Conclusion: We conclude that various dental plaque biofilm bacteria induce different cytokine response profiles in primary HGECs that may reflect their individual virulence or commensal status.Keywords
This publication has 45 references indexed in Scilit:
- Porphyromonas gingivalis induce apoptosis in human gingival epithelial cells through a gingipain-dependent mechanismBMC Microbiology, 2009
- Neutrophils rescue gingival epithelial cells from bacterial-induced apoptosisJournal of Leukocyte Biology, 2009
- The host cytokine response to Porphyromonas gingivalis is modified by gingipainsOral Microbiology and Immunology, 2008
- Interleukin-1β Modulates Proinflammatory Cytokine Production in Human Epithelial CellsInfection and Immunity, 2008
- P. gingivalis interactions with epithelial cellsFrontiers in Bioscience-Landmark, 2008
- Gingival Epithelial Cell Transcriptional Responses to Commensal and Opportunistic Oral Microbial SpeciesInfection and Immunity, 2007
- Innate Immune Response of Oral and Foreskin Keratinocytes: Utilization of Different Signaling Pathways by Various Bacterial SpeciesInfection and Immunity, 2004
- MAP Kinases in the Immune ResponseAnnual Review of Immunology, 2002
- Inducible Expression of Human β-Defensin 2 byFusobacterium nucleatumin Oral Epithelial Cells: Multiple Signaling Pathways and Role of Commensal Bacteria in Innate Immunity and the Epithelial BarrierInfection and Immunity, 2000
- Refractory Periodontitis: Mixed Infection with Bacteroides gingivalis and Other Unusual Bacteroides Species : A Case ReportThe Journal of Periodontology, 1988