Acute heat treatment improves insulin-stimulated glucose uptake in aged skeletal muscle
- 1 February 2011
- journal article
- research article
- Published by American Physiological Society in Journal of Applied Physiology
- Vol. 110 (2), 451-457
- https://doi.org/10.1152/japplphysiol.00849.2010
Abstract
Aging is associated with insulin resistance and decreased insulin-stimulated glucose uptake into skeletal muscle. Although the mechanisms underlying age-related insulin resistance are not clearly defined, impaired defense against inflammation and tissue oxidative stress are likely causes. Heat shock proteins (HSPs) have been shown to protect tissue from oxidative stress and inhibit the activation of stress kinases such as JNK, known to interfere with the insulin signaling pathway. While the induction of HSPs via chronic heat treatment has been shown to protect skeletal muscle from obesity-related insulin resistance, the ability of heat treatment to improve insulin action in aged skeletal muscle is not known. In the present study, one bout of in vivo heat treatment applied to 24-mo-old Fischer 344 rats improved insulin-stimulated glucose uptake after 24 h in slow-twitch soleus muscles. In vitro heat treatment applied to young (3-mo-old) and aged (24-mo-old) soleus muscles increased expression of HSP72 and inhibited anisomycin-induced activation of JNK. In contrast, heat treatment had no effect on p38 MAPK, a MAPK strongly activated with anisomycin. Prior inhibition of HSP72 transcription with the pharmacological inhibitor KNK437 eliminated the ability of heat treatment to blunt JNK activation. This suggests that the ability of heat treatment to inhibit JNK activation in skeletal muscle is dependent on increased HSP72 expression. In conclusion, an acute bout of heat treatment can increase insulin-stimulated glucose uptake in aged skeletal muscle, with the underlying mechanism likely to be HSP72-mediated JNK inhibition.Keywords
This publication has 58 references indexed in Scilit:
- Xenohormesis: health benefits from an eon of plant stress response evolutionCell Stress and Chaperones, 2010
- Heat Treatment Improves Glucose Tolerance and Prevents Skeletal Muscle Insulin Resistance in Rats Fed a High-Fat DietDiabetes, 2009
- Improvement of Insulin Sensitivity by a Novel Drug, BGP-15, in Insulin-resistant Patients: A Proof of Concept Randomized Double-blind Clinical TrialHormone and Metabolic Research, 2009
- Mild Electrical Stimulation with Heat Shock Ameliorates Insulin Resistance via Enhanced Insulin SignalingPLOS ONE, 2008
- HSP72 protects against obesity-induced insulin resistanceProceedings of the National Academy of Sciences of the United States of America, 2008
- Abnormal EKG stress test in rats with type 1 diabetes is deterred with low-intensity exercise programmeActa Diabetologica, 2006
- Reactive oxygen species have a causal role in multiple forms of insulin resistanceNature, 2006
- Manipulating heat shock protein expression in laboratory animalsMethods, 2005
- Attenuated HSP70 response in skeletal muscle of aged rats following contractile activityMuscle & Nerve, 2002
- Hot-Tub Therapy for Type 2 Diabetes MellitusThe New England Journal of Medicine, 1999