SOLUBLE THROMBOSPONDIN-1 SUPPRESSES T CELL PROLIFERATION AND ENHANCES IL-10 SECRETION BY ANTIGEN PRESENTING CELLS STIMULATED WITH PHYTOHEMAGGLUTININ
- 1 January 2001
- journal article
- Published by Informa UK Limited in Immunological Investigations
- Vol. 30 (2), 143-156
- https://doi.org/10.1081/imm-100104022
Abstract
Thrombospondin-1 (TSP-1) is different from other components of the extracellular matrix (ECM) regarding its production and distribution. TSP-1 is considered to be released in large quantity in inflammatory sites and exogenously added TSP-1 does not bind to preformed ECM but instead binds to cells. To define the physiological role of TSP-1 in the immune system, we studied the influence of TSP-1 on the in vitro culture of T cells and antigen-presenting cells (APCs) in the presence of phytohemagglutinin. By adding soluble TSP-1 to the culture, T cell proliferation was suppressed and anti-inflammatory cytokine IL-10 secretion by APCs was enhanced. The enhanced expression of IL-10 was also demonstrated at the mRNA level by RT-PCR using multiprimer kit for cytokines. The suppression of T cell proliferation and the enhancement of IL-10 secretion with soluble TSP-1 was inhibited by adding RGDS peptide or heparin. This result indicates that the effect of soluble TSP-1 may be caused by binding to its ligand(s) on T cells and/or APCs, resulting in transducing regulatory signals to the cells or disturbing appropriate interaction between T cells and APCs. We therefore propose that TSP-1 is an immunosuppressive modulator which may play a role in inflammatory sites.Keywords
This publication has 20 references indexed in Scilit:
- Unresponsiveness of Peripheral T Cells Induced by Apoptotic Bodies Derived from Autologous T CellsCellular Immunology, 1999
- STIMULATION OF G-CSF GENE EXPRESSION IN THE MACROPHAGE CELL LINE BY CONTACT WITH EXTRACELLULAR MATRIX PROTEINS AND A PRE-B LEUKAEMIA CELL LINECytokine, 1998
- Thrombospondin-1 Is a Major Activator of TGF-β1 In VivoCell, 1998
- Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF.JCI Insight, 1998
- Apoptosis observed in peripheral T lymphocytes from patients with chronic hepatitis BGastroenterology, 1996
- TCR/CD3 coupling to Fas-based cytotoxicity.The Journal of Experimental Medicine, 1995
- Endocytosis of Integrin (α5β1 (Fibronectin Receptor) of Mouse Peritoneal Macrophages in Vitro: An Immunoelectron Microscopic StudyJournal of Structural Biology, 1993
- Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding.The Journal of cell biology, 1993
- Thrombospondin sequence motif (CSVTCG) is responsible for CD36 bindingBiochemical and Biophysical Research Communications, 1992
- Regulated expression and binding of three VLA (β1) integrin receptors on T cellsNature, 1990