Insulin-Like Factor 3 Levels in Second-Trimester Amniotic Fluid
Open Access
- 1 October 2008
- journal article
- other
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 93 (10), 4048-4051
- https://doi.org/10.1210/jc.2008-0358
Abstract
Background: According to animal studies, the testicular Leydig cell hormone insulin-like factor 3 (Insl3) exerts a fundamental role in abdominal testis translocation, which occurs in the beginning of the second trimester in humans. Despite this, human prenatal INSL3 production has been poorly investigated. Methods: Amniotic fluid from 91 pregnant women undergoing amniocentesis was analyzed for INSL3 and testosterone (T) levels. Data were related to gestational age (15–25 wk) at amniocentesis and to sex (45 males and 48 females). Results: INSL3 was present in amniotic fluid from all but one of the investigated male fetuses (range: <0.02–0.36 ng/ml; mean ± sd: 0.12 ± 0.07), whereas the hormone was undetectable in the female fetuses. T was significantly higher in male (range: 0.54–1.71 nmol/liter; mean ± sd: 1.04 ± 0.30) as compared with in female amniotic fluid (range: 0.19–0.50 nmol/liter; mean ± sd: 0.34 ± 0.06) (P < 0.001). In males there was no correlation between INSL3 and T. A statistically borderline negative association was found between INSL3 and gestational age (P = 0.07), whereas the corresponding association was not significant for T (P = 0.12). In contrast, T in females correlated positively with gestational age (P = 0.02). Conclusions: INSL3 is clearly present in human male amniotic fluid in the second trimester, where abdominal testis translocation takes place. In contrast, the hormone is undetectable in female amniotic fluid. The prenatal presence of INSL3 supports the hypothesis that this hormone is essential for testicular descent in humans.Keywords
This publication has 19 references indexed in Scilit:
- Insulin-like factor 3 levels in amniotic fluid of human male fetusesHuman Reproduction, 2008
- Developmental Changes in Human Fetal Testicular Cell Numbers and Messenger Ribonucleic Acid Levels during the Second TrimesterJournal of Clinical Endocrinology & Metabolism, 2007
- Insulin-Like Factor 3 Levels in Cord Blood and Serum from Children: Effects of Age, Postnatal Hypothalamic-Pituitary-Gonadal Axis Activation, and CryptorchidismJournal of Clinical Endocrinology & Metabolism, 2007
- Cryptorchidism in common eutherian mammalsReproduction, 2007
- Peripheral INSL3 concentrations decline with age in a large population of Australian menInternational Journal of Andrology, 2006
- Changes in Serum Insulin-Like Factor 3 during Normal Male PubertyJournal of Clinical Endocrinology & Metabolism, 2006
- Insulin-Like Factor 3 Serum Levels in 135 Normal Men and 85 Men with Testicular Disorders: Relationship to the Luteinizing Hormone-Testosterone AxisJournal of Clinical Endocrinology & Metabolism, 2005
- Paracrine regulation of mammalian oocyte maturation and male germ cell survivalProceedings of the National Academy of Sciences of the United States of America, 2004
- LEYDIG INSULIN-LIKE HORMONE, GUBERNACULAR DEVELOPMENT AND TESTICULAR DESCENTJournal of Urology, 2001
- Cryptorchidism in mice mutant for Insl3Nature Genetics, 1999