The tomato 66.3-kD polyphenoloxidase gene: molecular identification and developmental expression.
- 1 February 1992
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 4 (2), 135-147
- https://doi.org/10.1105/tpc.4.2.135
Abstract
A gene coding for a polypeptide abundant in tomato floral meristems was isolated and shown to represent a tomato 66.3-kD polyphenoloxidase. Analysis of cDNA clones and a corresponding intronless genomic clone indicated that the plastid-bound 587-residue-long polypeptide, designated P2, contains two conserved copper-binding domains, similar to those found in fungal and mammalian tyrosinases. P2 transcripts and polypeptides are accumulated in the arrested floral primordia of the anantha mutant inflorescences and are equally abundant in primordia of wild-type flowers; the gene continues to be expressed at high levels in developing floral organs. In young expanding leaves, P2 protein is concentrated in palisade cells and in epidermal trichomes. Expression patterns of P2 in plant meristems permit molecular distinction between floral and vegetative primordia, and, in a companion study, comparison with dUTPase suggests that the two genes mark two alternative complementary developmental programs in the floral and vegetative meristems of the tomato plants.Keywords
This publication has 13 references indexed in Scilit:
- A meristem-related gene from tomato encodes a dUTPase: analysis of expression in vegetative and floral meristems.Plant Cell, 1992
- The MADS box gene family in tomato: temporal expression during floral development, conserved secondary structures and homology with homeotic genes from Antirrhinum and Arabidopsis.The Plant Journal, 1991
- Biosynthetic threonine deaminase gene of tomato: isolation, structure, and upregulation in floral organs.Proceedings of the National Academy of Sciences, 1991
- AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes.Genes & Development, 1991
- floricaula: A homeotic gene required for flower development in antirrhinum majusCell, 1990
- Phase Change and the Regulation of Shoot Morphogenesis in PlantsScience, 1990
- The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factorsNature, 1990
- Developmental programming of the shoot meristemCell, 1989
- Plants: Novel Developmental ProcessesScience, 1988
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970