Polymeric Drug for Antimicrobial Activity Studies: Synthesis and Characterization

Abstract

In this study, 2′,4-dichloro-5′-fluoro-1-ene-2-(4-hydroxyphenyl)phenone (EHP) was synthesized using 4-hydroxybenzaldehyde and 2, 4-dichloro-5-fluoro acetophenone (DFA); 4-[3-(2,4-dichloro-5-fluorophenyl)-3-oxoprop-1-en-1-yl]phenyl acrylate (EAP) was prepared by reacting EHP with acryloyl chloride. Poly(EAP) was prepared by solution polymerization and was characterized by IR, ¹H and ¹³C NMR. The molecular weights of the polymer (EAP) (M_w: 3900 and M_n: 3600) were characterized by gel permeation chromatographic. Thermal stability of the polymer was characterized by thermogravimetric analysis method. Antimicrobial activity of the DFA, EHP, EAP and poly(EAP) were tested on three microorganisms; Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Salmonella typhi ATCC 6539. The minimum concentration of the drug needed to inhibit the growth of the bacteria was estimated using a photometry assay.