Molecular force spectroscopy with a DNA origami–based nanoscopic force clamp
- 21 October 2016
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 354 (6310), 305-307
- https://doi.org/10.1126/science.aah5974
Abstract
Forces in biological systems are typically investigated at the single-molecule level with atomic force microscopy or optical and magnetic tweezers, but these techniques suffer from limited data throughput and their requirement for a physical connection to the macroscopic world. We introduce a self-assembled nanoscopic force clamp built from DNA that operates autonomously and allows massive parallelization. Single-stranded DNA sections of an origami structure acted as entropic springs and exerted controlled tension in the low piconewton range on a molecular system, whose conformational transitions were monitored by single-molecule Förster resonance energy transfer. We used the conformer switching of a Holliday junction as a benchmark and studied the TATA-binding protein–induced bending of a DNA duplex under tension. The observed suppression of bending above 10 piconewtons provides further evidence of mechanosensitivity in gene regulation.Keywords
Funding Information
- Deutsche Forschungsgemeinschaft (LI 1743/2-1, TI 329/6-1, GrK1952/1)
- Nanosystems Initiative Munich (SFB 1032 (TPA6), SFB 960 (TPA7))
- Braunschweig International Graduate School of Metrology
- European Commission (317110)
- European Research Council (336440)
- Boehringer Ingelheim Foundation
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