Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles
Open Access
- 4 June 2012
- journal article
- research article
- Published by Optica Publishing Group in Biomedical Optics Express
- Vol. 3 (7), 1492-1505
- https://doi.org/10.1364/boe.3.001492
Abstract
We present the implementation of a combined digital scanned light-sheet microscope (DSLM) able to work in the linear and nonlinear regimes under either Gaussian or Bessel beam excitation schemes. A complete characterization of the setup is performed and a comparison of the performance of each DSLM imaging modality is presented using in vivo Caenorhabditis elegans samples. We found that the use of Bessel beam nonlinear excitation results in better image contrast over a wider field of view.Keywords
This publication has 22 references indexed in Scilit:
- Fast, high-contrast imaging of animal development with scanned light sheet–based structured-illumination microscopyNature Methods, 2010
- A simple scanless two-photon fluorescence microscope using selective plane illuminationOptics Express, 2010
- Selective plane illumination microscopy techniques in developmental biologyDevelopment, 2009
- Quantitative in vivo imaging of entire embryos with Digital Scanned Laser Light Sheet Fluorescence MicroscopyCurrent Opinion in Neurobiology, 2008
- Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet MicroscopyScience, 2008
- Light sheet‐based fluorescence microscopy: More dimensions, more photons, and less photodamageHFSP Journal, 2008
- Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM)Optics Letters, 2007
- Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination MicroscopyScience, 2004
- Nonlinear magic: multiphoton microscopy in the biosciencesNature Biotechnology, 2003
- Laser scanning microscopyProceedings of the IEEE, 1982