Pathophysiological levels of the obesity related peptides resistin and ghrelin increase adhesion molecule expression on human vascular endothelial cells
- 1 October 2005
- journal article
- research article
- Published by Wiley in Clinical and Experimental Pharmacology and Physiology
- Vol. 32 (10), 839-844
- https://doi.org/10.1111/j.1440-1681.2005.04274.x
Abstract
1. In the present study, we sought to determine whether physiological or pathophysiological concentrations of obesity related peptides influence the key early atherogenic events of monocyte adhesion to endothelial cells and adhesion molecule expression using primary human cells. 2. Human umbilical vein endothelial cells were grown to confluence and human monocytes were obtained by elutriation. Adhesion was assessed by automated cell counting and cell adhesion molecule expression (E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1)) was assayed by ELISA. 3. Experimental conditions included untreated control, ghrelin (100, 150, 450 and 1350 pmol/L), resistin (15, 40 and 100 ng/mL) and combined leptin and insulin (combinations of 30 and 120 pmol/L insulin and 5, 50 and 500 ng/mL leptin). 4. Both resistin and ghrelin produced modest but significant increases in VCAM-1 expression (110 +/- 4 and 117 +/- 13% compared with controls, respectively; both P <or= 0.01). Ghrelin also increased ICAM-1 expression (119 +/- 17% of control; P <or= 0.01). 5. However, despite these increases in adhesion molecule expression, neither ghrelin nor resistin altered monocyte adhesion values. 6. Neither leptin nor insulin altered monocyte adhesion to endothelial cells or cell adhesion molecule expression. 7. Pathophysiologically relevant concentrations of ghrelin and resistin, within the range of concentrations exhibited by patients with anorexia nervosa or the Prader-Willi syndrome and type 2 diabetes, respectively, increase endothelial cell adhesion molecule expression, possibly contributing to increased atherosclerosis risk in such subjects.Keywords
This publication has 42 references indexed in Scilit:
- Loss of Meal-Induced Decrease in Plasma Ghrelin Levels in Patients with Anorexia NervosaJournal of Clinical Endocrinology & Metabolism, 2003
- Body Fat Mass and Macronutrient Intake in Relation to Circulating Soluble Leptin Receptor, Free Leptin Index, Adiponectin, and Resistin Concentrations in Healthy HumansJournal of Clinical Endocrinology & Metabolism, 2003
- Serum Ghrelin Levels Are Inversely Correlated with Body Mass Index, Age, and Insulin Concentrations in Normal Children and Are Markedly Increased in Prader-Willi SyndromeJournal of Clinical Endocrinology & Metabolism, 2003
- Plasma Resistin Levels in Patients with Type 1 and Type 2 Diabetes Mellitus and in Healthy ControlsHormone and Metabolic Research, 2002
- Does Leptin Cause Vascular Disease?Circulation, 2002
- Plasma Ghrelin Levels after Diet-Induced Weight Loss or Gastric Bypass SurgeryNew England Journal of Medicine, 2002
- Plasma Ghrelin Levels in Lean and Obese Humans and the Effect of Glucose on Ghrelin SecretionJournal of Clinical Endocrinology & Metabolism, 2002
- American Heart Association Call to Action: Obesity as a Major Risk Factor for Coronary Heart DiseaseCirculation, 1998
- Human macrophage-mediated oxidation of low-density lipoprotein is delayed and independent of superoxide productionBiochemical Journal, 1994
- The pathogenesis of atherosclerosis: a perspective for the 1990sNature, 1993