Validated methods for the quantification of biologically active constituents of poplar‐type propolis
- 1 July 2004
- journal article
- research article
- Published by Wiley in Phytochemical Analysis
- Vol. 15 (4), 235-240
- https://doi.org/10.1002/pca.777
Abstract
The validation of rapid, low-cost spectrophotometric procedures for the quantification of the three main groups of bioactive substances (flavones and flavonols, flavanones and dihydroflavonols, and total phenolics) in poplar-type propolis has been performed. A spectrophotometric assay based on the formation of an aluminium chloride complex was applied for the quantification of total flavones and flavonols using galangin as standard. Because of the high amount of flavanones and dihydroflavonols in “poplar type” propolis, the introduction of a distinct procedure for their quantification was considered of special significance and the DAB9 colorimetric method was applied for the purpose. Total phenolic content was measured by the Folin–Ciocalteu procedure using a mixture of pinocembrin and galangin as a reference. The procedures were validated using a model mixture of compounds representing the poplar-type propolis composition as found in previous studies. The accuracy (recovery) varied in the range 84–109%, and the relative standard deviation was 0.5–6.2%. The developed spectrophotometric procedures were applied to six poplar type propolis samples. The results were verified independently by a HPLC procedure. The two sets of results agreed satisfactory, as proven by Student's t-test. Copyright © 2004 John Wiley & Sons, Ltd.Keywords
This publication has 18 references indexed in Scilit:
- Chemical Composition of European Propolis: Expected and Unexpected ResultsZeitschrift für Naturforschung C, 2002
- Recent progress in pharmacological research of propolisPhytotherapy Research, 2001
- Egyptian Propolis: 1-Antimicrobial Activity and Chemical Composition of Upper Egypt PropolisZeitschrift für Naturforschung C, 2001
- Chemical Composition and Biological Activity of Propolis from Brazilian MeliponinaeZeitschrift für Naturforschung C, 2000
- Comparison of the free radical-scavenging activity of propolis from several regions of ArgentinaJournal of Ethnopharmacology, 2000
- Analysis of propolis: some parameters and procedures for chemical quality controlJournal of Apicultural Research, 1998
- The composition, active components and bacteriostatic activity of propolis in dieteticsJournal of Oil & Fat Industries, 1994
- Determination of phenolics from propolis by capillary gas chromatographyJournal of Chromatography A, 1992
- Synthesis of Natural Esters of Substituted Cinnamic AcidsJournal of Natural Products, 1990
- The Composition and Plant Origins of Propolis: A Report of Work at OxfordBee World, 1990