Stimulation of respiration by methylene blue in rat liver mitochondria
Open Access
- 21 July 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 412 (1), 157-160
- https://doi.org/10.1016/s0014-5793(97)00767-9
Abstract
The effect of methylene blue on isolated rat liver mitochondria in the presence and absence of chloroacetaldehyde was investigated. Fatty acid oxidation was inhibited by chloroacetaldehyde and subsequently stimulated by methylene blue. Assessment of tightly coupled mitochondria revealed decreasing respiratory control ratios induced by increasing concentrations of methylene blue and methylene blue provoked mitochondrial swelling. In uncoupled mitochondria, methylene blue promoted a concentration‐dependent stimulation of respiration. These findings provide evidence that methylene blue, the redox dye currently used as an antidote for encephalopathy associated with alkylating chemotherapy, uncouples oxidative phosphorylation and acts as an electron transfer mediator to stimulate mitochondrial respiration.This publication has 26 references indexed in Scilit:
- Ifosfamide/MesnaDrugs, 1991
- 2-Chloroacetaldehyde and 2-chloroacetal are potent inhibitors of DNA synthesis in animal cellsBiochemical and Biophysical Research Communications, 1990
- Pharmacology of Reye SyndromeClinical Neuropharmacology, 1987
- Reye's syndrome: Current conceptsHepatology, 1987
- MODIFYING INTRACELLULAR REDOX BALANCE: AN APPROACH TO IMPROVING THERAPEUTIC INDEXThe Lancet, 1985
- 2-Chloroacetaldehyde, a metabolite of cyclophosphamide in the ratXenobiotica, 1983
- Mutagenicity of vinyl chloride, chloroethyleneoxide, chloroacetaldehyde and chloroethanolBiochemical and Biophysical Research Communications, 1975
- METHYLENE-BLUE IN ALCOHOL-INDUCED HYPOGLYCEMIAThe Lancet, 1965
- ENCEPHALOPATHY AND FATTY DEGENERATION OF THE VISCERA A DISEASE ENTITY IN CHILDHOODThe Lancet, 1963
- Croonian Lecture - Lethal synthesisProceedings of the Royal Society of London. Series B - Biological Sciences, 1952