Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum
Open Access
- 16 September 2008
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 105 (37), 13730-13735
- https://doi.org/10.1073/pnas.0806100105
Abstract
Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in P. falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.This publication has 33 references indexed in Scilit:
- The ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum stabilizes spectrin tetramers and suppresses further invasionBlood, 2007
- Illuminating Plasmodium falciparum-infected red blood cellsTrends in Parasitology, 2007
- Effect of plasmodial RESA protein on deformability of human red blood cells harboring Plasmodium falciparumProceedings of the National Academy of Sciences of the United States of America, 2007
- Molecular Factors and Biochemical Pathways Induced by Febrile Temperature in Intraerythrocytic Plasmodium falciparum ParasitesInfection and Immunity, 2007
- Diffraction phase and fluorescence microscopyOptics Express, 2006
- A role for the Plasmodium falciparum RESA protein in resistance against heat shock demonstrated using gene disruptionMolecular Microbiology, 2005
- The pathogenic basis of malariaNature, 2002
- Molecular Maps of Red Cell Deformation: Hidden Elasticity and in Situ ConnectivityScience, 1994
- Spectrin involvement in a 40°C structural transition of the red blood cell membraneJournal of Cellular Biochemistry, 1986
- Plasmodium falciparum Maturation Abolishes Physiologic Red Cell DeformabilityScience, 1984