Effect of Sulforaphane and 5-Aza-2’-Deoxycytidine on Melanoma Cell Growth
Open Access
- 27 June 2019
- Vol. 6 (3), 71
- https://doi.org/10.3390/medicines6030071
Abstract
Background: UV exposure-induced oxidative stress is implicated as a driving mechanism for melanoma. Increased oxidative stress results in DNA damage and epigenetic dysregulation. Accordingly, we explored whether a low dose of the antioxidant sulforaphane (SFN) in combination with the epigenetic drug 5-aza-2’-deoxycytidine (DAC) could slow melanoma cell growth. SFN is a natural bioactivated product of the cruciferous family, while DAC is a DNA methyltransferase inhibitor. Methods: Melanoma cell growth characteristics, gene transcription profiles, and histone epigenetic modifications were measured after single and combination treatments with SFN and DAC. Results: We detected melanoma cell growth inhibition and specific changes in gene expression profiles upon combinational treatments with SFN and DAC, while no significant alterations in histone epigenetic modifications were observed. Dysregulated gene transcription of a key immunoregulator cytokine—C-C motif ligand 5 (CCL-5)—was validated. Conclusions: These results indicate a potential combinatorial effect of a dietary antioxidant and an FDA-approved epigenetic drug in controlling melanoma cell growth.Keywords
This publication has 86 references indexed in Scilit:
- Genome-wide screen of promoter methylation identifies novel markers in melanomaGenome Research, 2009
- Cellular Responses to Cancer Chemopreventive Agent D,L-Sulforaphane in Human Prostate Cancer Cells Are Initiated by Mitochondrial Reactive Oxygen SpeciesPharmaceutical Research, 2009
- Oral sulforaphane increases Phase II antioxidant enzymes in the human upper airwayClinical Immunology, 2009
- TopHat: discovering splice junctions with RNA-SeqBioinformatics, 2009
- UV and pigmentation: molecular mechanisms and social controversiesPigment Cell & Melanoma Research, 2008
- High-throughput functional annotation and data mining with the Blast2GO suiteNucleic Acids Research, 2008
- Genetic determinants of hair, eye and skin pigmentation in EuropeansNature Genetics, 2007
- Lightening up the UV response by identification of the arylhydrocarbon receptor as a cytoplasmatic target for ultraviolet B radiationProceedings of the National Academy of Sciences of the United States of America, 2007
- Cruciferous vegetables and human cancer risk: epidemiologic evidence and mechanistic basisPharmacological Research, 2007
- Long-distance combinatorial linkage between methylation and acetylation on histone H3 N terminiProceedings of the National Academy of Sciences of the United States of America, 2007