An Alkaloid Extract of Evanta, Traditionally Used as Anti‐leishmania Agent in Bolivia, Inhibits Cellular Proliferation and Interferon‐γ Production in Polyclonally Activated Cells
Open Access
- 8 February 2009
- journal article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 69 (3), 251-258
- https://doi.org/10.1111/j.1365-3083.2008.02219.x
Abstract
Traditional medicine and scientific studies have shown that the raw extract of Evanta [Galipea longiflora, Angostura longiflora (Krause) Kallunki] exhibits anti-leishmanial activity. We hypothesized that the healing observed when using this plant might not only be due to the direct action on the parasite, but possibly to a parallel effect on the host immune response to the parasite involved in the healing process. We show here that an alkaloid extract of Evanta (AEE) directly killed the parasite already at a dose of 10 microg/ml, but at this low concentration, AEE did not have a major effect on viability and proliferation of eukaryotic cells. The whole extract was also found to be stronger than 2-phenylquinoline, the most prominent alkaloid in AEE. AEE was not directly stimulating B or T cells or J774 macrophages. However, it interfered with the activation of both mouse and human T cells, as revealed by a reduction of in vitro cellular proliferation and interferon-gamma (IFN-gamma) production. The effect was more evident when the cells were pretreated with AEE and subsequently stimulated with the polyclonal T-cell activators Concanavalin A and anti-CD3. Taken together, our results suggest that Evanta have a direct leishmanicidal effect and due to the effect on IFN-gamma production it might contribute to control the chronic inflammatory reaction that characterize Leishmania infection pathology, but in vivo studies are necessary to corroborate this finding.Keywords
This publication has 30 references indexed in Scilit:
- Can interferon-γ and interleukin-10 balance be associated with severity of human Leishmania (Viannia) braziliensis infection?Clinical and Experimental Immunology, 2007
- Differential immune regulation of activated T cells between cutaneous and mucosal leishmaniasis as a model for pathogenesisParasite Immunology, 2007
- Immunomodulatory effects of caffeine: Friend or foe?Pharmacology & Therapeutics, 2006
- Mucosal Leishmaniasis Patients Display an Activated Inflammatory T‐cell Phenotype Associated with a Nonbalanced Monocyte PopulationScandinavian Journal of Immunology, 2005
- Glucantime® par voie parentérale : bénéfice versus toxicitéMedecine Et Maladies Infectieuses, 2005
- Chloroquine induces activation of nuclear factor‐κB and subsequent expression of pro‐inflammatory cytokines by human astroglial cellsJournal of Neurochemistry, 2003
- The immunology of susceptibility and resistance to Leishmania major in miceNature Reviews Immunology, 2002
- Latent Activity of Curcumin against Leishmaniasis in Vitro.Biological & Pharmaceutical Bulletin, 2002
- Immunochemotherapy in American cutaneous leishmaniasis: immunological aspects before and after treatmentMemórias do Instituto Oswaldo Cruz, 2001
- Antiprotozoal activity of quinoline alkaloids isolated from Galipea longiflora, a Bolivian plant used as a treatment for cutaneous leishmaniasisPhytotherapy Research, 1994