Differential Expression of KAI1 Metastasis Suppressor Protein in Renal Cell Tumor Histological Subtypes
- 1 May 2009
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of Urology
- Vol. 181 (5), 2305-2311
- https://doi.org/10.1016/j.juro.2009.01.003
Abstract
The similar appearance of renal tumor histological subtypes can complicate differential diagnoses. This problem is most notable for the chromophobe subtype of renal cell carcinoma, which can be histologically indistinguishable from oncocytoma with investigational molecular markers failing to provide reliable differentiation. KAI1 is a metastasis suppressor gene whose expression correlates inversely with the metastatic potential of most solid tumor cancer types. We tested the hypothesis that KAI1 is differentially expressed among renal tumor histological subtypes. Immunohistochemical staining for KAI1 protein was performed in 152 nephrectomy specimens, including 48 clear cell, 35 papillary and 31 chromophobe renal cell carcinoma samples, 28 oncocytomas and 10 tumor-free kidneys. Staining was scored as none/minimal, low, moderate or high. KAI1 mRNA levels were compared by quantitative reverse transcriptase-polymerase chain reaction in an additional 22 chromophobe renal cell carcinoma and oncocytoma samples. In all 10 tumor-free kidneys KAI1 protein was detected exclusively in distal tubule cell membranes. Of the tumor specimens KAI1 protein was absent in all papillary renal cell carcinoma specimens. It was present in only 1 of 48 clear cell renal cell carcinomas (2%) and 2 of 28 oncocytomas (7%) but only at low levels. In contrast, 27 of 31 chromophobe renal cell carcinoma specimens (87%) expressed KAI1 protein, most at moderate or high levels. The diagnostic accuracy of KAI1 immunostaining for discerning chromophobe renal cell carcinoma from oncocytoma was 90% with similar results observed at the RNA level. KAI1 is an accurate biomarker for chromophobe renal cell carcinoma that may aid in the diagnostic differentiation of chromophobe renal cell carcinoma from oncocytoma. It remains to be determined whether KAI1 expression contributes to the low metastatic potential of chromophobe renal cell carcinoma.Keywords
This publication has 15 references indexed in Scilit:
- Expression Analysis of Kidney-specific Cadherin in a Wide Spectrum of Traditional and Newly Recognized Renal Epithelial Neoplasms: Diagnostic and Histogenetic ImplicationsThe American Journal of Surgical Pathology, 2007
- Gene Expression Profiling Separates Chromophobe Renal Cell Carcinoma from Oncocytoma and Identifies Vesicular Transport and Cell Junction Proteins as Differentially Expressed GenesClinical Cancer Research, 2006
- Validation of tissue microarray technology in endometrioid cancer of the endometriumJournal of Clinical Pathology, 2006
- Expression of Kidney-Specific Cadherin in Chromophobe Renal Cell Carcinoma and Renal OncocytomaAmerican Journal of Clinical Pathology, 2006
- Caveolin-1 Immunohistochemical Analysis in Differentiating Chromophobe Renal Cell Carcinoma From Renal OncocytomaAmerican Journal of Clinical Pathology, 2006
- CD70: A NEW TUMOR SPECIFIC BIOMARKER FOR RENAL CELL CARCINOMAJournal of Urology, 2005
- C-kit expression in renal oncocytomas and chromophobe renal cell carcinomasHuman Pathology, 2005
- Expression of kidney-specific cadherin distinguishes chromophobe renal cell carcinoma from renal oncocytomaHuman Pathology, 2005
- Expression of Alpha-Methylacyl-CoA Racemase in Papillary Renal Cell CarcinomaThe American Journal of Surgical Pathology, 2004
- Metastasis Suppression: The Evolving Role of Metastasis Suppressor Genes for Regulating Cancer Cell Growth at the Secondary SiteJournal of Urology, 2003