Heat shock proteins as key biological targets of the marine natural cyclopeptide perthamide C
- 27 January 2012
- journal article
- Published by Royal Society of Chemistry (RSC) in Molecular BioSystems
- Vol. 8 (5), 1412-1417
- https://doi.org/10.1039/c2mb05507d
Abstract
Linking bioactive compounds to their cellular targets is a central challenge in chemical biology. Herein we report the mode of action of perthamide C, a natural cyclopeptide isolated from the marine sponge Theonella swinhoei. Through an emerging mass spectrometry-based chemical proteomics approach, Heat Shock Protein 90 and Glucose Regulated Protein 94 were identified as key targets of perthamide C and this evidence has been validated using surface plasmon resonance. The ability of perthamide C to influence heat shock protein-mediated cell apoptosis revealed that this marine metabolite could be a good candidate for the development of a lead compound with therapeutic applications based on apoptosis modulation.Keywords
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