Pathogenic Role of Macrophages in Intradermal Infection of Methicillin-Resistant Staphylococcus aureus in Thermally Injured Mice
- 1 October 2010
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 78 (10), 4311-4319
- https://doi.org/10.1128/iai.00642-10
Abstract
Intradermal infection of methicillin-resistant Staphylococcus aureus (MRSA) in burned mice was pathogenically analyzed. An abscess was formed in normal mice intradermally infected with 10 8 CFU/mouse of MRSA, and all of these mice survived after the infection; however, abscess formation was not demonstrated to occur in burned mice similarly exposed to the pathogen, and all of these mice died within 5 days of infection. In burned mice, MRSA infected at the burn site intradermal tissues spread quickly throughout the whole body, while in normal mice, the pathogen remained localized at the infection site. Macrophages (Mφ) isolated from the infection site tissues of normal mice produced interleukin-12 (IL-12) but not IL-10 and were characterized as M1Mφ. These M1Mφ were not isolated from the infection site tissues of burned mice. When normal-mouse infection site tissue Mφ were adoptively transferred to burned mice at the MRSA infection site, an abscess formed, and the infection did not develop into sepsis. In contrast, an abscess did not form and sepsis developed in normal mice that were inoculated with burned-mouse infection site tissue Mφ. These Mφ produced IL-10 but not IL-12 and were characterized as M2Mφ. These results indicate that abscess formation is a major mechanism of host resistance against intradermal MRSA infection. M1Mφ in the tissues surrounding the infection site play a pivotal role in abscess formation; however, the abscess is not formed in burned mice where M2Mφ predominate. M2Mφ have been described as inhibitor cells for Mφ conversion from resident Mφ to M1Mφ.Keywords
This publication has 33 references indexed in Scilit:
- Emerging Infections in BurnsSurgical Infections, 2009
- Tolerance and M2 (alternative) macrophage polarization are related processes orchestrated by p50 nuclear factor κBProceedings of the National Academy of Sciences, 2009
- Trends in the Incidence of Methicillin‐ResistantStaphylococcus aureusInfection in Children’s Hospitals in the United StatesClinical Infectious Diseases, 2009
- Macrophage elastase kills bacteria within murine macrophagesNature, 2009
- Immune cell subsets in necrotizing fasciitis: an immunohistochemical analysisVirchows Archiv, 2009
- Interleukin‐10: new perspectives on an old cytokineImmunological Reviews, 2008
- Biochemical and functional characterization of three activated macrophage populationsJournal of Leukocyte Biology, 2006
- Burn Wound InfectionsClinical Microbiology Reviews, 2006
- Sepsis in pediatric burn patientsPediatric Critical Care Medicine, 2005
- Interleukin-12 and the regulation of innate resistance and adaptive immunityNature Reviews Immunology, 2003