Recurrent Uveitis in Horses: Vitreal Examinations with Ultrastructural Detection of Leptospires
- 22 May 2007
- journal article
- Published by Wiley in Journal of Veterinary Medicine Series A
- Vol. 54 (5), 270-275
- https://doi.org/10.1111/j.1439-0442.2007.00921.x
Abstract
This study documents the examination of 17 horses (both sexes, 3–18 years old) suffering from spontaneous equine recurrent uveitis (ERU). Vitreal samples obtained by pars plana vitrectomy were examined macroscopically and ultrastructurally, and in most cases also by cultural examination, by microscopic agglutination test (MAT) and by polymerase chain reaction. In 24% (4/17) of the animals, ultrastructural examination by electron microscopy revealed intact leptospiral bacteria in the vitreous. The leptospires were detected freely in the vitreous and also incorporated by a phagocyte. They were surrounded by a rim of proteinaceous material which was reduced around a phagocytosed leptospira. Ninety-four per cent (16/17) of the vitreal samples presented significant antibody levels in the MAT, mostly against leptospiral serovar Grippotyphosa. Seventy-five per cent (9/12) of bacterial culture examinations were positive for leptospira. Polymerase chain reaction was positive in all (16/16) examinations performed. Our findings support previous reports suggesting that leptospires play an important role in the pathogenesis of ERU. Interestingly, this study found leptospires after secondary and later acute episodes. A persistent leptospiral infection is therefore suggested as the cause of ERU.Keywords
This publication has 19 references indexed in Scilit:
- Effect of vaccination against leptospirosis on the frequency, days to recurrence and progression of disease in horses with equine recurrent uveitisVeterinary Ophthalmology, 2005
- Classification of Leptospira from the Eyes of Horses Suffering from Recurrent UveitisJournal of Veterinary Medicine, Series B, 2004
- Autoimmune uveitis induced by molecular mimicry of peptides from rotavirus, bovine casein and retinal S‐antigenEuropean Journal of Immunology, 2003
- LeptospirosisOcular Immunology and Inflammation, 1999
- Comparison of Polymerase Chain Reaction with Microagglutination Test and Culture for Diagnosis of LeptospirosisThe Journal of Infectious Diseases, 1995
- Biochemical analysis by SDS-PAGE and Western blotting of the antigenic relationship between Leptospira and equine ocular tissuesVeterinary Immunology and Immunopathology, 1992
- Pars Plicata in Equine Recurrent UveitisVeterinary Pathology, 1990
- Studies on equine recurrent uveitis. II: The role of infection withLeptospira interrogansserovarpomonaCurrent Eye Research, 1985
- PHAGOCYTOSIS AND ITS SIGNIFICANCE IN LEPTOSPIRAL INFECTIONImmunology & Cell Biology, 1964
- Electron microscopic studies of the morphology of pathogenic spirochætesThe Journal of Pathology and Bacteriology, 1955