Effects of ascorbic acid and a-tocopherol on arsenic-induced oxidative stress
- 1 December 2002
- journal article
- research article
- Published by SAGE Publications in Human & Experimental Toxicology
- Vol. 21 (12), 675-680
- https://doi.org/10.1191/0960327102ht307oa
Abstract
Arsenic is an ubiquitous element in the environment causing oxidative burst in the exposed individuals leading to tissue damage. Antioxidants have long been known to reduce the free radical-mediated oxidative stress. Therefore, the present study was designed to determine whether supplementation of a-tocopherol (400 mg/kg body weight) and ascorbic acid (200 mg/kg body weight) to arsenic-intoxicated rats (100 ppm in drinking water) for 30 days affords protection against the oxidative stress caused by the metalloid. The arsenic-treated rats showed elevated levels of lipid peroxide, decreased levels of non-enzymatic antioxidants and activities of enzymatic antioxidants. Administration of a-tocopherol and ascorbic acid to arsenic-exposed rats showed a decrease in the level of lipid peroxidation (LPO) and enhanced levels of total sulfhydryls, reduced glutathione, ascorbic acid and a-tocopherol and so do the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase to near normal. These findings suggest thata-tocopherol and ascorbic acid prevent LPO and protect the antioxidant system in arsenic-intoxicated rats.Keywords
This publication has 28 references indexed in Scilit:
- Role of Vitamin E in Ascorbate-Dependent Protein Thiol Oxidation in Rat Liver Endoplasmic ReticulumArchives of Biochemistry and Biophysics, 2001
- Mitochondrial Uptake and Recycling of Ascorbic AcidArchives of Biochemistry and Biophysics, 2001
- Induction of oxyradicals by arsenic: Implication for mechanism of genotoxicityProceedings of the National Academy of Sciences of the United States of America, 2001
- DNA damage in arsenite- and cadmium-treated bovine aortic endothelial cellsFree Radical Biology & Medicine, 2000
- Antioxidants in Human Health and DiseaseAnnual Review of Nutrition, 1996
- Dose-response relationship between peripheral vascular disease and ingested inorganic arsenic among residents in blackfoot disease endemic villages in TaiwanAtherosclerosis, 1996
- Ingested Inorganic Arsenic and Prevalence of Diabetes MellitusAmerican Journal of Epidemiology, 1994
- Cancer potential in liver, lung, bladder and kidney due to ingested inorganic arsenic in drinking waterBritish Journal of Cancer, 1992
- Assay for lipid peroxides in animal tissues by thiobarbituric acid reactionAnalytical Biochemistry, 1979
- Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liverBiochimica et Biophysica Acta (BBA) - General Subjects, 1979