Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property
- 12 December 2006
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 103 (50), 18998-19003
- https://doi.org/10.1073/pnas.0608155103
Abstract
Signaling through the Notch pathway regulates multiple aspects of development. The vertebrate retina allows an investigation of the basis for these various effects, because the major cell types of the retina arise from a common progenitor that expresses Notch1. The Notch pathway was constitutively activated in distinct populations of retinal cells during development. Prolonged Notch activity in progenitor cells maintained cells in the progenitor state without perturbing temporal identity, promoting early progenitor characteristics early in development and late progenitor characteristics later in development. Eventually, constitutive Notch activation led these cells to acquire characteristics of glial and stem cells. In contrast, reactivating the Notch pathway in newly postmitotic retinal cells promoted mature glial cell formation in a subset of cells. These data suggest that prolonged Notch activity does not disrupt the normal progression of progenitor temporal states, and that down-regulating or overcoming Notch activity is required for proper formation of both neuronal and glial cell fates.Keywords
This publication has 53 references indexed in Scilit:
- Neural stem cell properties of Müller glia in the mammalian retina: Regulation by Notch and Wnt signalingDevelopmental Biology, 2006
- Notch1 functions to suppress cone-photoreceptor fate specification in the developing mouse retinaDevelopment, 2006
- Notch 1 inhibits photoreceptor production in the developing mammalian retinaDevelopment, 2006
- Wnt2b inhibits differentiation of retinal progenitor cells in the absence of Notch activity by downregulating the expression of proneural genesDevelopment, 2005
- Evidence for an Expansion-Based Temporal Shh Gradient in Specifying Vertebrate Digit IdentitiesCell, 2004
- Genomic Analysis of Mouse Retinal DevelopmentPLoS Biology, 2004
- Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporterDevelopmental Biology, 2004
- Vertebrate neural cell-fate determination: Lessons from the retinaNature Reviews Neuroscience, 2001
- Notch Signaling: Cell Fate Control and Signal Integration in DevelopmentScience, 1999
- Cyclin D1 provides a link between development and oncogenesis in the retina and breastCell, 1995