A Simple and Rapid Method for DNA Isolation from Xylophagous Insects
Open Access
- 6 December 2010
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 11 (12), 5056-5064
- https://doi.org/10.3390/ijms11125056
Abstract
Published methods to isolate DNA from insects are not always effective in xylophagous insects because they have high concentrations of phenolics and other secondary plant compounds in their digestive tracts. A simple, reliable and labor-effective cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method for isolation of high quality DNA from xylophagous insects is described. This method was successfully applied to PCR and restriction analysis, indicating removal of common inhibitors. DNA isolated by the CTAB-PVP method could be used in most molecular analyses.This publication has 23 references indexed in Scilit:
- A Simple Method for DNA Extraction from Mature Date Palm Leaves: Impact of Sand Grinding and Composition of Lysis BufferInternational Journal of Molecular Sciences, 2010
- The phylogeographical clade trade: tracing the impact of human‐mediated dispersal on the colonization of northern Europe by the oak gallwasp Andricus kollariMolecular Ecology, 2007
- Amplification facilitators and multiplex PCR: Tools to overcome PCR-inhibition in DNA-gut-content analysis of soil-living invertebratesSoil Biology and Biochemistry, 2006
- The ecology of forest insect invasions and advances in their managementCanadian Journal of Forest Research, 2006
- The role of herbivory by wood‐boring insects in mangrove ecosystems in BelizeOikos, 2002
- The Current State Of Insect Molecular Systematics: A Thriving Tower of BabelAnnual Review of Entomology, 2000
- Trypanosomatidae:PhytomonasDetection in Plants and Phytophagous Insects by PCR Amplification of a Genus-Specific Sequence of the Spliced Leader GeneExperimental Parasitology, 1999
- A simple and efficient method for DNA extraction from grapevine cultivars andVitis speciesPlant Molecular Biology Reporter, 1994
- Isolation of high-molecular-weight DNA from insectsAnalytical Biochemistry, 1990
- Insect Herbivore Relationship to the State of the Host Plant: Biotic Regulation of Ecosystem Nutrient Cycling through Ecological SuccessionOikos, 1981