Specific regulation of immediate early genes by patterned neuronal activity
- 1 August 1993
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 35 (5), 459-467
- https://doi.org/10.1002/jnr.490350502
Abstract
Electrical activity shapes development of the nervous system, presumably in part by regulating gene expression. A set of regulatory genes, immediate early genes (IEGs), which are responsive to a number of extrinsic cellular stimuli have been proposed to play a role in coupling such activity to gene expression. Using a semiquantitative polymerase chain reaction assay, we show that in dissociated mouse dorsal root ganglion neurons the expression of two IEGs, c-fos and nur/77, is differentially sensitive to patterns of electrical stimulation. Differences in c-fos activation did not correlate with the peak intracellular calcium [Ca++]i produced by the different stimulation patterns or with residual [Ca++]i following stimulation. However, the net increase in [Ca++]i (calcium time integral) was greater for the pulsed stimulus that activated c-fos (6 impulses/min), compared to the ineffective stimulus (12 impulses/2 min). This system of genes seems suited to mediating the coupling between electrical activity and other functional genes.This publication has 24 references indexed in Scilit:
- Accommodation of mouse DRG growth cones to electrically induced collapse: Kinetic analysis of calcium transients and set‐point theoryJournal of Neurobiology, 1993
- A simple and rapid method to analyze specific mRNAs from few cells in a semi-quantitative way using the polymerase chain reaction.Genome Research, 1991
- CREB: a Ca 2+ -Regulated Transcription Factor Phosphorylated by Calmodulin-Dependent KinasesScience, 1991
- Synchronous Bursts of Action Potentials in Ganglion Cells of the Developing Mammalian RetinaScience, 1991
- Stimulus-Transcription Coupling in the Nervous System: Involvement of the Inducible Proto-Oncogenes fos and junAnnual Review of Neuroscience, 1991
- Membrane depolarization and calcium induce c-fos transcription via phosphorylation of transcription factor CREBNeuron, 1990
- The regulation and function of c-fos and other immediate early genes in the nervous systemNeuron, 1990
- Intracellular calibration of the fluorescent calcium indicator Fura-2Cell Calcium, 1990
- Long-term potentiation and the induction of c-fos mRNA and proteins in the dentate gyrus of unanesthetized ratsNeuroscience Letters, 1989
- Stimulus-transcription coupling in neurons: role of cellular immediate-early genesTrends in Neurosciences, 1989