Mesenchymal stem cells from adipose and bone marrow promote angiogenesis via distinct cytokine and protease expression mechanisms
- 21 November 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Angiogenesis
- Vol. 14 (1), 47-59
- https://doi.org/10.1007/s10456-010-9194-9
Abstract
Using a fibrin-based angiogenesis model, we have established that there is no canonical mechanism used by endothelial cells (ECs) to degrade the surrounding extracellular matrix (ECM), but rather the set of proteases used is dependent on the mural cells providing the angiogenic cues. Mesenchymal stem cells (MSCs) originating from different tissues, which are thought to be phenotypically similar, promote angiogenesis through distinct mechanisms. Specifically, adipose-derived stem cells (ASCs) promote utilization of the plasminogen activator-plasmin axis by ECs as the primary means of vessel invasion and elongation in fibrin. Matrix metalloproteinases (MMPs) serve a purpose in regulating capillary diameter and possibly in stabilizing the nascent vessels. These proteolytic mechanisms are more akin to those involved in fibroblast-mediated angiogenesis than to those in bone marrow-derived stem cell (BMSC)-mediated angiogenesis. In addition, expression patterns of angiogenic factors such as urokinase plasminogen activator (uPA), hepatocyte growth factor (HGF), and tumor necrosis factor alpha (TNFα) were similar for ASC and fibroblast-mediated angiogenesis, and in direct contrast to BMSC-mediated angiogenesis. The present study illustrates that the nature of the heterotypic interactions between mural cells and endothelial cells depend on the identity of the mural cell used. Even MSCs which are shown to behave phenotypically similar do not stimulate angiogenesis via the same mechanisms.Keywords
This publication has 44 references indexed in Scilit:
- Stromal regulation of vessel stability by MMP14 and TGFβDisease Models & Mechanisms, 2010
- Biomimetic hydrogels with pro-angiogenic propertiesBiomaterials, 2010
- Mesenchymal cells stimulate capillary morphogenesis via distinct proteolytic mechanismsExperimental Cell Research, 2010
- Bioartificial matrices for therapeutic vascularizationProceedings of the National Academy of Sciences, 2009
- TNF-α drives remodeling of blood vessels and lymphatics in sustained airway inflammation in miceJCI Insight, 2009
- Endothelial cell lumen and vascular guidance tunnel formation requires MT1-MMP–dependent proteolysis in 3-dimensional collagen matricesBlood, 2009
- A Perivascular Origin for Mesenchymal Stem Cells in Multiple Human OrgansCell Stem Cell, 2008
- Secretion of Angiogenic and Antiapoptotic Factors by Human Adipose Stromal CellsCirculation, 2004
- Yield of human adipose-derived adult stem cells from liposuction aspiratesCytotherapy, 2004
- Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based TherapiesTissue Engineering, 2001