A cluster of 11 CBF transcription factors is located at the frost tolerance locus Fr-A m 2 in Triticum monococcum
- 16 December 2005
- journal article
- research article
- Published by Springer Science and Business Media LLC in Molecular Genetics and Genomics
- Vol. 275 (2), 193-203
- https://doi.org/10.1007/s00438-005-0076-6
Abstract
Due to the adverse effects of cold temperatures on winter wheat, frost tolerance is an important trait for breeding programs in regions with severe winters. Frost tolerance locus Fr-A m 2 was recently discovered in diploid wheat (Triticum monococcum L.). This locus was mapped as a QTL on chromosome 5Am in the same region as a QTL for the level of transcription of the cold-regulated gene COR14b at 15°C. A CBF transcription factor was mapped in the center of these two overlapping QTLs. However, since the CBF gene family in wheat has numerous members, it was possible that multiple CBF genes were present at Fr-A m 2. To investigate this possibility we initiated a systematic characterization of the CBF family in T. monococcum. Here we report the molecular characterization of thirteen TmCBF genes. Nine of them were numbered according to the closest barley HvCBF gene, and the other four that have no clear barley orthologues were assigned numbers TmCBF15 to TmCBF18. TmCBF5 and TmCBF18 were mapped on T. monococcum chromosomes 7Am and 6Am, respectively, and are thus not candidates for the Fr-A m 2 gene. The remaining eleven TmCBF genes are clustered at the Fr-A m 2 locus within five different Bacterial Artificial Chromosome (BAC) clones. These BACs were mapped using a high-density map and recombination events were found between most BACs. Lines carrying these recombination events will be useful to identify which of the CBF genes is responsible for the differences in frost tolerance between the T. monococcum parental lines at the Fr-A m 2 locus.Keywords
This publication has 55 references indexed in Scilit:
- Regulation by Vrn-1/Fr-1 chromosomal intervals of CBF-mediated Cor/Lea gene expression and freezing tolerance in common wheatJournal of Experimental Botany, 2005
- Structure and Expression Profile of the Arabidopsis PHO1 Gene Family Indicates a Broad Role in Inorganic Phosphate HomeostasisPlant Physiology, 2004
- TaVRT-1, a Putative Transcription Factor Associated with Vegetative to Reproductive Transition in CerealsPlant Physiology, 2003
- Two loci on wheat chromosome 5A regulate the differential cold-dependent expression of the cor14b gene in frost-tolerant and frost-sensitive genotypesMolecular Genetics and Genomics, 2000
- Physical mapping of the Vrn-A1 and Fr1 genes on chromosome 5A of wheat using deletion linesTheoretical and Applied Genetics, 1999
- The Interaction between Cold and Light Controls the Expression of the Cold-Regulated Barley Gene cor14b and the Accumulation of the Corresponding Protein1Plant Physiology, 1999
- Construction and characterization of a bacterial artificial chromosome (BAC) library for the A genome of wheatGenome, 1999
- AnArabidopsisGene Family Encoding DRE/CRT Binding Proteins Involved in Low-Temperature-Responsive Gene ExpressionBiochemical and Biophysical Research Communications, 1998
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994