Membrane current noise in dark‐adapted and light‐adapted isolated retinal rods of the larval tiger salamander

Abstract

1. Low-frequency light-sensitive membrane current noise in isolated rod photoreceptors of the larval tiger salamander was recorded using suction electrodes, in the dark, and during light adaptation by backgrounds or by bleaching visual pigment. 2. In background light, noise variance increases and then decreases. For rods desensitized to similar levels by bleaching visual pigment, the noise variance either does not change (weak adaptation) or decreases (with stronger adaptation). 3. The power spectral density of the current noise in dark-adapted rods shows a component with half-power cut-off frequency at about 0.1 Hz, attributed to spontaneous single events and continuous noise from dark phosphodiesterase activity. A second component, with half-power cut-off frequency at about 1 Hz, may be due to slow components in the light-sensitive channel gating. 4. The power spectral density of the noise in background light is dominated by noise generated by the background. Background light adapts at least the first component of the noise seen in dark-adapted cells. For cells desensitized by bleaching, light adaptation of both components of the dark-adapted noise is observed. 5. The results confirm that the low-frequency noise in dark-adapted cells arises from the transduction mechanism of the rod, in that both components can be light adapted, and show that, for rods permanently desensitized by bleaching, the desensitization is not due to the presence of active visual pigment molecules similar to those produced by background light.