Prevalence of Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. in Seafood Products Using Multiplex Polymerase Chain Reaction
- 1 February 2012
- journal article
- Published by Mary Ann Liebert Inc in Foodborne Pathogens & Disease
- Vol. 9 (2), 108-112
- https://doi.org/10.1089/fpd.2011.0989
Abstract
Although several etiological agents can be transmitted through seafood consumption, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. are considered among the most important pathogens in terms of public health and disease. In this study, multiplex polymerase chain reaction (PCR), as a rapid and cost-effective method, was used to determine the prevalence of these pathogens in 245 samples of raw/fresh, frozen, and ready-to-eat (RTE) seafood products marketed in Iran. The prevalence of L. monocytogenes in raw/fresh fish and shrimp samples was 1.4%, whereas 2.9% of the raw/fresh fish and 7.1% of the shrimp samples were contaminated with V. parahaemolyticus. No contamination with L. monocytogenes and V. parahaemolyticus was found in frozen and RTE seafood products. The prevalence of S. aureus was found to be higher than other investigated pathogens. S. aureus was detected in 5% of the raw/fresh samples of fish and shrimp, 17.5% of the frozen, and 12.3% of the RTE samples. Further, our findings indicate that 2.9% of the fish samples, 4.3% of the shrimp samples, and 1.5% of the RTE samples were contaminated with Salmonella spp. Owing to the potential hazard of these pathogenic bacteria, multiplex PCR can provide a rapid and cost-effective method for the surveillance of these pathogens in seafood products.Keywords
This publication has 22 references indexed in Scilit:
- Vibrios as causal agents of zoonosesVeterinary Microbiology, 2010
- Simultaneous detection of Escherichia coli O175:H7, Salmonella spp., and Listeria monocytogenes by multiplex PCRFood Control, 2009
- Bacterial pathogens in fresh, smoked and salted Iranian fishFood Control, 2006
- The Handbook of Microbiological Media for the Examination of FoodPublished by Taylor & Francis Ltd ,2006
- Detection ofVibrio parahaemolyticusin cockle (Anadara granosa) by PCRFEMS Microbiology Letters, 2005
- Simultaneous detection of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella strains by real-time PCRInternational Journal of Food Microbiology, 2002
- The role of seafood in bacterialfoodborne diseasesMicrobes and Infection, 2000
- Enterotoxigenic Escherichia coli and Staphylococcus aureus in fish and seafood from the southern region of BrazilInternational Journal of Food Microbiology, 1994
- Presence, detection and growth of Listeria monocytogenes in seafoods: a reviewInternational Journal of Food Microbiology, 1994
- Foodborne Diseases in the United States Associated with Meat and PoultryJournal of Food Protection, 1980