In Vivo Imaging of Glial Activation after Unilateral Labyrinthectomy in the Rat: A [18F]GE180-PET Study
Open Access
- 11 December 2017
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Neurology
Abstract
The functional relevance of reactive gliosis for recovery from acute unilateral vestibulopathy is unknown. In the present study, glial activation was visualized in vivo by [18F]GE180-PET in a rat model of unilateral labyrinthectomy (UL) and compared to behavioral vestibular compensation (VC) overtime. 14 Sprague-Dawley rats underwent a UL by transtympanic injection of bupivacaine/arsenilate, 14 rats a SHAM UL (injection of normal saline). Glial activation was depicted with [18F]GE180-PET and ex vivo autoradiography at baseline and 7, 15, 30 days after UL/SHAM UL. Postural asymmetry and nystagmus were registered at 1, 2, 3, 7, 15, 30 days after UL/SHAM UL. Signs of vestibular imbalance were found only after UL, which significantly decreased until days 15 and 30. In parallel, [18F]GE180-PET and ex vivo autoradiography depicted glial activation in the ipsilesional vestibular nerve and nucleus on days 7 and 15 after UL. Correlation analysis revealed a strong negative association of [18F]GE180 uptake in the ipsilesional vestibular nucleus on day 7 with the rate of postural recovery (R = −0.90, p < 0.001), suggesting that glial activation accelerates VC. In conclusion, glial activation takes place in the ipsilesional vestibular nerve and nucleus within the first 30 days after UL in the rat and can be visualized in vivo by [18F]GE180-PET.Keywords
Funding Information
- Bundesministerium für Bildung und Forschung (01 EO 0901)
This publication has 42 references indexed in Scilit:
- Mechanisms of vestibular compensation: recent advancesCurrent Opinion in Otolaryngology & Head and Neck Surgery, 2010
- Improved method for combination of immunocytochemistry and Nissl stainingJournal of Neuroscience Methods, 2009
- Etifoxine improves peripheral nerve regeneration and functional recoveryProceedings of the National Academy of Sciences of the United States of America, 2008
- Role of the commissural inhibitory system in vestibular compensation in the ratThe Journal of Physiology, 2008
- Translocator protein 18 kDa (TSPO): Molecular sensor of brain injury and repairPharmacology & Therapeutics, 2008
- Role of the peripheral benzodiazepine receptor in sensory neuron regenerationMolecular and Cellular Neuroscience, 2005
- Axonal injury‐dependent induction of the peripheral benzodiazepine receptor in small‐diameter adult rat primary sensory neuronsEuropean Journal of Neuroscience, 2004
- Long-Term Plasticity of Ipsilesional Medial Vestibular Nucleus Neurons After Unilateral LabyrinthectomyJournal of Neurophysiology, 2003
- Evidence for Reactive Astrocytes in Rat Vestibular and Cochlear Nuclei Following Unilateral Inner Ear LesionEuropean Journal of Neuroscience, 1996
- Experimentally (Atoxyl) Induced Ampullar Degeneration and Damage to the Maculae UtriculiActa Oto-Laryngologica, 1977