Identification of a soluble form of a ligand for the lymphocyte homing receptor.
Open Access
- 1 November 1992
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 176 (5), 1415-1419
- https://doi.org/10.1084/jem.176.5.1415
Abstract
Lymphocytes are engaged in constant trafficking from the blood into secondary lymphoid tissues, such as peripheral lymph nodes (PLN), mesenteric lymph nodes (MLN), and Peyer's patches (PP). The initial step in this process is the binding of lymphocytes to high endothelial venules (HEV), and in the case of trafficking of cells to the PLN, it is required that they bear the L-selectin surface receptor. Using a chimeric protein, combining the extracellular domains of L-selectin with a human immunoglobulin (Ig) G1 Fc region (L-selectin-IgG), we have probed the expression of ligands for this receptor on HEV and in cell lysates. Two sulfated glycoproteins of 50 and 90 kD have been identified in lysates from PLN and MLN, but not PP. Here we show that the 50-kD molecule is secreted in organ cultures in vitro and is present in the blood of normal animals. Indeed, normal serum inhibits lymphocyte binding to HEV by approximately 50% in an in vitro assay. This inhibitory activity can be removed by passage of the serum over an L-selectin-IgG column and has a molecular mass of approximately 50 kD. We speculate on the possible reasons for secretion of a homing receptor ligand.Keywords
This publication has 18 references indexed in Scilit:
- An endothelial ligand for L-Selectin is a novel mucin-like moleculeCell, 1992
- Identification of a carbohydrate-based endothelial ligand for a lymphocyte homing receptor.The Journal of cell biology, 1991
- A homing receptor-IgG chimera as a probe for adhesive ligands of lymph node high endothelial venules.The Journal of cell biology, 1990
- The Mel 14 antibody binds to the lectin domain of the murine peripheral lymph node homing receptor.The Journal of cell biology, 1990
- Demonstration that a lectin-like receptor (gp90MEL) directly mediates adhesion of lymphocytes to high endothelial venules of lymph nodes.The Journal of cell biology, 1989
- Neutrophil Mac-1 and MEL-14 Adhesion Proteins Inversely Regulated by Chemotactic FactorsScience, 1989
- Homing Receptors and Vascular Addressins: Cell Adhesion Molecules that Direct Lymphocyte TrafficImmunological Reviews, 1989
- Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes.The Journal of cell biology, 1988
- Major histocompatibility antigens: The human (HLA-A,-B,-C) and murine (H-2K, H-2D) class I moleculesCell, 1981
- Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ‐specific determinants on high endothelial venulesEuropean Journal of Immunology, 1980