The H-NS protein silences thepypregulatory network ofYersinia enterocoliticaand is involved in controlling biofilm formation
Open Access
- 30 January 2013
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 340 (1), 41-48
- https://doi.org/10.1111/1574-6968.12073
Abstract
Horizontal gene transfer plays an important role in bacterial evolution. DNA acquired by horizontal gene transfer has to be incorporated into existing regulatory networks. The histone-like nucleoid structuring protein H-NS acts as a silencer of horizontally acquired genes to avoid potential damage. However, specific regulators can overcome H-NS repression, resulting in the integration of newly acquired genes into existing regulatory networks. Here, we analyzed the influence of H-NS on the transcription of the Yersinia enterocolitica hreP gene and its regulators pypA, pypB, and pypC by establishing a dominant-negative H-NS version. Using transcriptional fusions and electrophoretic mobility shift assays, we show that H-NS silences hreP, pypA, pypB, and pypC by direct interactions. While the H-NS antagonist RovA activates pypC, it has no effect on pypA and pypB. Furthermore, H-NS affects biofilm formation in Y. enterocolitica.Keywords
This publication has 30 references indexed in Scilit:
- A Natural System of Chromosome Transfer in Yersinia pseudotuberculosisPLoS Genetics, 2012
- Structural basis for recognition of AT-rich DNA by unrelated xenogeneic silencing proteinsProceedings of the National Academy of Sciences, 2011
- H-NS forms a superhelical protein scaffold for DNA condensationProceedings of the National Academy of Sciences, 2010
- Transcriptional Activation of the tad Type IVb Pilus Operon by PypB in Yersinia enterocoliticaJournal of Bacteriology, 2010
- Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ E and H-NSJournal of Bacteriology, 2010
- A divalent switch drives H-NS/DNA-binding conformations between stiffening and bridging modesGenes & Development, 2010
- A Regulatory Network Controls Expression of the In Vivo-Expressed HreP Protease of Yersinia enterocoliticaJournal of Bacteriology, 2009
- H-NS RepressesinvTranscription inYersinia enterocoliticathrough Competition with RovA and Interaction with YmoAJournal of Bacteriology, 2006
- Application of Comparative Phylogenomics To Study the Evolution ofYersinia enterocoliticaand To Identify Genetic Differences Relating to PathogenicityJournal of Bacteriology, 2006
- The Complete Genome Sequence and Comparative Genome Analysis of the High Pathogenicity Yersinia enterocolitica Strain 8081PLoS Genetics, 2006