The structure of porcine protegrin genes
- 17 July 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 368 (2), 197-202
- https://doi.org/10.1016/0014-5793(95)00633-k
Abstract
We cloned the genes of three protegrins, a family of cathelin‐associated antimicrobial peptides originally isolated from porcine leukocytes. Each gene comprised 4 exons and 3 introns, wherein Exon I encoded the signal sequence and the first 37 amino acids of cathelin, Exons II and III contained 36 and 24 additional cathelin residues and Exon IV contained the final two cathelin residues followed by the protegrin sequence. This quadripartite gene structure helps explain how structurally diverse antimicrobial peptides can be expressed on common, cathelin‐containing precursors. Southern blot probed with an oligonucleotide specific for protegrin genes suggested that several identical or nearly identical protegrin genes were densely clustered in the pig chromosome.Keywords
This publication has 26 references indexed in Scilit:
- Identification of a new member of the protegrin family by cDNA cloningFEBS Letters, 1994
- Chemical synthesis and biological activity of a novel antibacterial peptide deduced from a pig myeloid cDNAFEBS Letters, 1994
- Molecular cloning of a putative homolog of proline/arginine‐rich antibacterial peptides from porcine bone marrowFEBS Letters, 1993
- A cDNA Derived from Pig Bone Marrow Cells Predicts a Sequence Identical to the Intestinal Antibacterial Peptide PR-39Biochemical and Biophysical Research Communications, 1993
- A Novel cDNA Sequence Encoding a Pig Leukocyte Antimicrobial Peptide with a Cathelin-like Pro-sequenceBiochemical and Biophysical Research Communications, 1993
- Primary structure of three cationic peptides from porcine neutrophilsFEBS Letters, 1993
- cDNA sequence analysis of an antibiotic dodecapeptide from neutrophilsFEBS Letters, 1992
- CDNA cloning of the neutrophil bactericidal peptide indolicidinBiochemical and Biophysical Research Communications, 1992
- Complementary DNA sequence of rabbit CAP18—A unique lipopolysaccharide binding proteinBiochemical and Biophysical Research Communications, 1991
- Primary structure of a new cysteine proteinase inhibitor from pig leucocytesFEBS Letters, 1989