Magnetic nanoparticle biosensors
Top Cited Papers
- 5 April 2010
- journal article
- review article
- Published by Wiley in WIREs Nanomedicine and Nanobiotechnology
- Vol. 2 (3), 291-304
- https://doi.org/10.1002/wnan.84
Abstract
One of the major challenges in medicine is the rapid and accurate measurement of protein biomarkers, cells, and pathogens in biological samples. A number of new diagnostic platforms have recently been developed to measure biomolecules and cells with high sensitivity that could enable early disease detection or provide valuable insights into biology at the systems level. Most biological samples exhibit negligible magnetic susceptibility; therefore, magnetic nanoparticles have been used for diverse applications including biosensing, magnetic separation, and thermal ablation therapy. This review focuses on the use of magnetic nanoparticles for detection of biomolecules and cells based on magnetic resonance effects using a general detection platform termed diagnostic magnetic resonance (DMR). DMR technology encompasses numerous assay configurations and sensing principles, and to date magnetic nanoparticle biosensors have been designed to detect a wide range of targets including DNA/mRNA, proteins, enzymes, drugs, pathogens, and tumor cells. The core principle behind DMR is the use of magnetic nanoparticles as proximity sensors that modulate the spin‐spin relaxation time of neighboring water molecules, which can be quantified using clinical MRI scanners or benchtop nuclear magnetic resonance (NMR) relaxometers. Recently, the capabilities of DMR technology were advanced considerably with the development of miniaturized, chip‐based NMR (µNMR) detector systems that are capable of performing highly sensitive measurements on microliter sample volumes and in multiplexed format. With these and future advances in mind, DMR biosensor technology holds considerable promise to provide a high‐throughput, low‐cost, and portable platform for large scale molecular and cellular screening in clinical and point‐of‐care settings. WIREs Nanomed Nanobiotechnol 2010 2 291–304 This article is categorized under: Diagnostic Tools > Biosensing Diagnostic Tools > In Vitro Nanoparticle-Based SensingKeywords
This publication has 64 references indexed in Scilit:
- Ultrasensitive Detection of Bacteria Using Core–Shell Nanoparticles and an NMR‐Filter SystemAngewandte Chemie, 2009
- Implantable diagnostic device for cancer monitoringBiosensors and Bioelectronics, 2009
- Nanoparticle−Target Interactions Parallel Antibody−Protein InteractionsAnalytical Chemistry, 2009
- 18F Labeled Nanoparticles for in Vivo PET-CT ImagingBioconjugate Chemistry, 2009
- Surface-Enhanced Raman Scattering Tags for Rapid and Homogeneous Detection of Circulating Tumor Cells in the Presence of Human Whole BloodJournal of the American Chemical Society, 2008
- Chip–NMR biosensor for detection and molecular analysis of cellsNature Medicine, 2008
- Electrode Chemistry Yields a Nanoparticle-Based NMR Sensor for CalciumLangmuir, 2008
- Sensitive NMR Sensors Detect Antibodies to InfluenzaAngewandte Chemie, 2008
- Spin valve sensors for ultrasensitive detection of superparamagnetic nanoparticles for biological applicationsSensors and Actuators A: Physical, 2006
- Mass spectrometry-based proteomicsNature, 2003