Basophils Promote Tumor Rejection via Chemotaxis and Infiltration of CD8+ T Cells
- 15 January 2017
- journal article
- Published by American Association for Cancer Research (AACR) in Cancer Research
- Vol. 77 (2), 291-302
- https://doi.org/10.1158/0008-5472.can-16-0993
Abstract
Elevated numbers of regulatory T-cells (Tregs) in patient tumors are known to inhibit efficient anti-tumor T-cell responses. To study the mechanisms controlling tumor rejection, we assessed different mouse models for Treg depletion. In Foxp3DTR knock-in mice, about 99% Treg depletion was achieved, resulting in complete rejection of transplanted HCmel12 melanomas in a CD8+ T cell-dependent way. In contrast, about 90% Treg depletion obtained in BAC transgenic Foxp3.LuciDTR4 mice failed to induce complete rejection of HCmel12 melanomas, demonstrating that residual Tregs were able to control CD8+ T cell responses against the tumor. 99% Treg depletion provoked drastic changes in the tumor microenvironment, such as strong infiltration of CD8+ T cells and basophils. Intratumoral basophils enhanced CD8+ T cell infiltration via production of chemokines CCL3 and CCL4; antibody-based blocking of these chemokines inhibited CD8+ T cell infiltration. Therapeutic induction of basophilia by IL-3/anti-IL-3 antibody complexes, combined with transfer of CD8 T-cells, resulted in enhanced T-cell infiltration and tumor rejection. Our study identifies a critical role basophils play in tumor rejection, and that this role can be exploited for therapeutic intervention.Keywords
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Funding Information
- Wilhelm Sander-Stiftung (2009.022.2)
This publication has 48 references indexed in Scilit:
- T-Regulatory Cells: Key Players in Tumor Immune Escape and AngiogenesisCancer Research, 2012
- TSLP promotes interleukin-3-independent basophil haematopoiesis and type 2 inflammationNature, 2011
- Selective ablation of basophils in mice reveals their nonredundant role in acquired immunity against ticksJCI Insight, 2010
- Dendritic Cells Support Homeostatic Expansion of Foxp3+ Regulatory T Cells in Foxp3.LuciDTR MiceThe Journal of Immunology, 2010
- Cutting Edge: Basophils Are Transiently Recruited into the Draining Lymph Nodes during Helminth Infection via IL-3, but Infection-Induced Th2 Immunity Can Develop without Basophil Lymph Node Recruitment or IL-3The Journal of Immunology, 2009
- MHC class II–dependent basophil–CD4+ T cell interactions promote TH2 cytokine–dependent immunityNature Immunology, 2009
- Basophils function as antigen-presenting cells for an allergen-induced T helper type 2 responseNature Immunology, 2009
- IL-18 and IL-33 elicit Th2 cytokines from basophils via a MyD88- and p38α-dependent pathwayJournal of Leukocyte Biology, 2009
- IL-3 Induces Basophil Expansion In Vivo by Directing Granulocyte-Monocyte Progenitors to Differentiate into Basophil Lineage-Restricted Progenitors in the Bone Marrow and by Increasing the Number of Basophil/Mast Cell Progenitors in the SpleenPublished by The American Association of Immunologists ,2009
- Human Basophils Secrete IL-3: Evidence of Autocrine Priming for Phenotypic and Functional Responses in Allergic DiseasePublished by The American Association of Immunologists ,2009