Liquid chromatographic–electrospray tandem mass spectrometric determination of bisoprolol in human plasma
- 12 February 2007
- journal article
- research article
- Published by Wiley in Biomedical Chromatography
- Vol. 21 (5), 508-513
- https://doi.org/10.1002/bmc.785
Abstract
An analytical method for the determination of bisoprolol in human plasma has been developed based on liquid chromatography–tandem mass spectrometry (LC–MS/MS). The analyte and internal standard (IS) diphenhydramine were cleaned up by protein precipitation with acetonitrile, reconstituted in mobile phase and separated by reversed‐phase high‐performance liquid chromatography (HPLC) using methanol:10 mm ammonium acetate:formic acid (70:30:0.1 v/v/v) as mobile phase. Detection was carried out by multiple reaction monitoring (MRM) on an LC–MS/MS system and was completed within 2.5 min. The assay was linear over the range 0.5–100 ng/mL with a limit of quantitation (LOQ) of 0.5 ng/mL. The intra‐ and inter‐day precision levels were within 5.54 and 9.95%, respectively, while the accuracy was in the range 89.4–113%. This method has been utilized in a pharmacokinetic study, where healthy volunteers were treated with an oral dose of 5 mg bisoprolol. Copyright © 2007 John Wiley & Sons, Ltd.Keywords
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