Effect of Adiponectin Gene Polymorphisms on Circulating Adiponectin and Insulin Resistance Indexes in Women with Polycystic Ovary Syndrome
Open Access
- 1 February 2005
- journal article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 51 (2), 416-423
- https://doi.org/10.1373/clinchem.2004.043109
Abstract
Background: We examined the possible association of adiponectin gene polymorphisms with polycystic ovary syndrome (PCOS) and their influence on serum adiponectin and insulin resistance indexes in Greek women with PCOS. Methods: We genotyped samples from 100 women with PCOS characterized with respect to body mass index (BMI), glucose and insulin concentrations during an oral glucose tolerance test (OGTT), lipid profile, and serum adiponectin concentrations and from 140 healthy controls for the 45T>G and 276G>T polymorphisms in the adiponectin gene. Results: The distributions of genotypes and alleles of both polymorphisms were no different in women with PCOS and controls, indicating that the individual polymorphisms are not associated with increased risk for PCOS. However, the two polymorphisms were found to be associated with insulin resistance indexes among women with PCOS and to influence adiponectin production. In particular, carriers of the TG genotype at position +45 had greater hyperinsulinemia, as estimated by the area under the curve for insulin (AUCinsulin) during the OGTT, than those with the TT genotype (P <0.05), and this was independent of age and BMI. In addition, women with PCOS with the GG or GT genotypes at position +276 had a higher BMI (P = 0.01) and greater AUCinsulin (P = 0.01) than carriers of the TT genotype. The latter genotype was found less frequently among overweight/obese women with PCOS than in normal-weight individuals (P = 0.002). In addition, the presence of the GG or GT genotype was associated with lower serum adiponectin than the TT genotype, independent of age, BMI, and insulin concentrations (P = 0.03). Serum adiponectin was negatively correlated with serum triglycerides and insulin resistance indexes and positively with HDL-cholesterol. Conclusions: Adiponectin gene polymorphisms at positions +45 and +276 are not associated with PCOS. However, these genomic variants may influence production of adiponectin and the metabolic variables related to insulin resistance/metabolic syndrome in patients with PCOS.Keywords
This publication has 33 references indexed in Scilit:
- Polycystic ovary syndrome: Syndrome XX?Trends in Endocrinology & Metabolism, 2003
- Serum adiponectin levels in women with polycystic ovary syndromeHuman Reproduction, 2003
- Prevalence and Predictors of Coronary Artery Calcification in Women with Polycystic Ovary SyndromeJournal of Clinical Endocrinology & Metabolism, 2003
- Adiponectin Levels in Women with Polycystic Ovary SyndromeJournal of Clinical Endocrinology & Metabolism, 2003
- The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesityNature Medicine, 2001
- Adipose Tissue as an Endocrine OrganTrends in Endocrinology & Metabolism, 2000
- Plasma Concentrations of a Novel, Adipose-Specific Protein, Adiponectin, in Type 2 Diabetic PatientsArteriosclerosis, Thrombosis, and Vascular Biology, 2000
- Paradoxical Decrease of an Adipose-Specific Protein, Adiponectin, in ObesityBiochemical and Biophysical Research Communications, 1999
- A Novel Serum Protein Similar to C1q, Produced Exclusively in AdipocytesJournal of Biological Chemistry, 1995
- The genetics of polycystic ovary syndromeAmerican Journal Of Medicine, 1995