Comparative study of photothermolysis of cancer cells with nuclear-targeted or cytoplasm-targeted gold nanospheres: continuous wave or pulsed lasers
- 1 January 2010
- journal article
- research article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 15 (5), 058002-058002-7
- https://doi.org/10.1117/1.3486538
Abstract
We conduct a comparative study on the efficiency and cell death pathways of continuous wave (cw) and nanosecond pulsed laser photothermal cancer therapy using gold nanospheres delivered to either the cytoplasm or nucleus of cancer cells. Cytoplasm localization is achieved using arginine-glycine-aspartate peptide modified gold nanospheres, which target integrin receptors on the cell surface and are subsequently internalized by the cells. Nuclear delivery is achieved by conjugating the gold nanospheres with nuclear localization sequence peptides originating from the simian virus. Photothermal experiments show that cell death can be induced with a single pulse of a nanosecond laser more efficiently than with a cw laser. When the cw laser is applied, gold nanospheres localized in the cytoplasm are more effective in inducing cell destruction than gold nanospheres localized at the nucleus. The opposite effect is observed when the nanosecond pulsed laser is used, suggesting that plasmonic field enhancement of the nonlinear absorption processes occurs at high localization of gold nanospheres at the nucleus. Cell death pathways are further investigated via a standard apoptosis kit to show that the cell death mechanisms depend on the type of laser used. While the cw laser induces cell death via apoptosis, the nanosecond pulsed laser leads to cell necrosis. These studies add mechanistic insight to gold nanoparticle-based photothermal therapy of cancer.Keywords
This publication has 46 references indexed in Scilit:
- Plasmonic photothermal therapy (PPTT) using gold nanoparticlesLasers in Medical Science, 2007
- Calculated Absorption and Scattering Properties of Gold Nanoparticles of Different Size, Shape, and Composition: Applications in Biological Imaging and BiomedicineThe Journal of Physical Chemistry B, 2006
- Cancer Cell Imaging and Photothermal Therapy in the Near-Infrared Region by Using Gold NanorodsJournal of the American Chemical Society, 2006
- Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destructionProceedings of the National Academy of Sciences of the United States of America, 2005
- Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidanceProceedings of the National Academy of Sciences of the United States of America, 2003
- Optical Properties and Ultrafast Dynamics of Metallic NanocrystalsAnnual Review of Physical Chemistry, 2003
- Some Interesting Properties of Metals Confined in Time and Nanometer Space of Different ShapesAccounts of Chemical Research, 2001
- Shape and size dependence of radiative, non-radiative and photothermal properties of gold nanocrystalsInternational Reviews in Physical Chemistry, 2000
- Spectral Properties and Relaxation Dynamics of Surface Plasmon Electronic Oscillations in Gold and Silver Nanodots and NanorodsThe Journal of Physical Chemistry B, 1999
- Tumour ablation by laser in general surgeryLasers in Medical Science, 1990