Comparison of Mycosis IC/F and Plus Aerobic/F Media for Diagnosis of Fungemia by the Bactec 9240 System
Open Access
- 1 February 2004
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (2), 773-777
- https://doi.org/10.1128/jcm.42.2.773-777.2004
Abstract
Fungemia is associated with a high mortality rate. We compared the performance of the Mycosis IC/F selective fungal medium and the Plus Aerobic/F standard bacteriological medium for the diagnosis of fungemia on the Bactec 9240 automatic system. We retrospectively analyzed 550 blood culture pairs composed of one Mycosis IC/F vial and one Plus Aerobic/F vial, drawn in 187 patients with fungemia. The positivity rate by vial was significantly higher on Mycosis IC/F medium than on Plus Aerobic/F medium (88.0% versus 74.9%, P < 0.0001). The positivity rate for fungus detection on Plus Aerobic/F medium fell to 26.9% when bacteria were present in the same vial. The positivity rate by patient was also significantly higher on Mycosis IC/F medium than on Plus Aerobic/F medium (92.5% versus 75.9%, P < 0.0001). A marked superiority of Mycosis IC/F medium was demonstrated for diagnosis of Candida glabrata fungemia (31 of 31, 100%, versus 18 of 31, 58.1%, P < 0.0001). The mean detection time was significantly shorter on Mycosis IC/F medium than on Plus Aerobic/F medium (28.9 ± 22.2 h versus 36.5 ± 24.6 h, P < 0.0001). The mean time saving was 8.8 h for Candida albicans and 43.7 h for C. glabrata . Mycosis IC/F medium enabled more sensitive and earlier diagnosis, particularly for the two strains most frequently responsible for fungemia, C. albicans and C. glabrata , and also in the event of the concomitant presence of both yeasts and bacteria. In patients with risk factors, it would thus appear to be sensible to draw a Mycosis IC/F vial in addition to the standard bacteriological vials.Keywords
This publication has 16 references indexed in Scilit:
- Use of simulated blood cultures for time to detection comparison between BacT/ALERT™ and BACTEC™ 9240 blood culture systemsDiagnostic Microbiology and Infectious Disease, 2002
- Comparison of Aerobic Standard Medium with Specific Fungal Medium for Detecting Fusarium Spp. in Blood CulturesEuropean Journal of Clinical Microbiology & Infectious Diseases, 2002
- Controlled Comparison of BacT/ALERT FAN Aerobic Medium and BACTEC Fungal Blood Culture Medium for Detection of FungemiaJournal of Clinical Microbiology, 2001
- Nosocomial Bloodstream Infections in United States Hospitals: A Three‐Year AnalysisClinical Infectious Diseases, 1999
- National Surveillance of Nosocomial Blood Stream Infection Due to Species of Candida Other than Candida albicans: Frequency of Occurrence and Antifungal Susceptibility in the SCOPE ProgramDiagnostic Microbiology and Infectious Disease, 1998
- Use of simulated blood cultures to compare a specific fungal medium with a standard microorganism medium for yeast detectionEuropean Journal of Clinical Microbiology & Infectious Diseases, 1998
- The Prevalence of Nosocomial Infection in Intensive Care Units in EuropeJAMA, 1995
- Nosocomial Candidemia: Risk Factors and Attributable MortalityClinical Infectious Diseases, 1995
- Hospital-acquired candidemia. The attributable mortality and excess length of stayArchives of Internal Medicine, 1988
- Hospital-Acquired CandidemiaArchives of Internal Medicine, 1988