Use of Monoclonal Anti-mouse Immunoglobulin to Detect Mouse Antibodies
- 1 January 1981
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 1 (1), 5-11
- https://doi.org/10.1089/hyb.1.1981.1.5
Abstract
Rat monoclonal antibodies specific for mouse kappa light chains and mouse γ heavy chains have been generated. These rat monoclonal antibodies have been biosynthetically labelled with 35S methionine. The free label was dialyzed from the medium and, without further purification, the medium containing the radioactive monoclonal antibody was used in a radioimmunoassay to screen the sera of the immunized animals and hybridomas for specific mouse antibodies of the IgG class.Keywords
This publication has 7 references indexed in Scilit:
- Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens*Immunological Reviews, 1979
- Allogeneic carrier-specific enhancement of hapten-specific secondary B-cell responses.The Journal of Experimental Medicine, 1976
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- A simplified method for cyanogen bromide activation of agarose for affinity chromatographyAnalytical Biochemistry, 1974
- Cloning of mouse myeloma cells and detection of rare variantsJournal of Cellular Physiology, 1972
- Affinity labeling of a phosphorylcholine binding mouse myeloma proteinBiochemistry, 1972
- Light Chain EvolutionPublished by Cold Spring Harbor Laboratory ,1967