Antifungal Chemical Compounds Identified Using a C. elegans Pathogenicity Assay
Open Access
- 2 February 2007
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLoS Pathogens
- Vol. 3 (2), e18
- https://doi.org/10.1371/journal.ppat.0030018
Abstract
Candida spp. are among the most significant causes of nosocomial infections, and disseminated candidiasis continues to have an attributable mortality rate of over 25%. For this reason, we have developed a liquid media assay using the model nematode Caenorhabditis elegans as a model organism for Candida infection. The worms are infected on solid media lawns and then moved to pathogen-free liquid media. Unless antifungal compounds are added to the wells, the majority of worms die within 3–4 d. This model is similar to the infection process in humans, in that Candida cells are able to produce filaments, which are essential for the infection process in humans. We used this pathogen model to create a semi-automated, high-throughput screen using C. elegans to evaluate the antifungal effectiveness of many types of chemical compounds. Through this process, we have identified three compounds that we show have varying degrees of antifungal activity in C. elegans, in vitro, and in mice.Keywords
This publication has 51 references indexed in Scilit:
- Critical Role of Bcr1-Dependent Adhesins in C. albicans Biofilm Formation In Vitro and In VivoPLoS Pathogens, 2006
- Identification of novel antimicrobials using a live-animal infection modelProceedings of the National Academy of Sciences of the United States of America, 2006
- Cryptococcus neoformans Gene Involved in Mammalian Pathogenesis Identified by a Caenorhabditis elegans Progeny-Based ApproachInfection and Immunity, 2005
- Candida Infections of Medical DevicesClinical Microbiology Reviews, 2004
- Candida biofilmsCurrent Opinion in Microbiology, 2002
- The filamentation pathway controlled by the Efg1 regulator protein is required for normal biofilm formation and development inCandida albicansFEMS Microbiology Letters, 2002
- A Conserved p38 MAP Kinase Pathway in Caenorhabditis elegans Innate ImmunityScience, 2002
- Estimating the Cost of Nosocomial Candidemia in the United StatesClinical Infectious Diseases, 2001
- Candida albicans hyphal formation and virulence: is there a clearly defined role?Trends in Microbiology, 1998
- Using the Nematode Caenorhabditis Elegans To Predict Mammalian Acute Lethality To Metallic SaltsToxicology and Industrial Health, 1988