Construction and properties of a triprotein containing the high-affinity nickel transporter ofAlcaligenes eutrophus
- 20 May 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 283 (1), 109-112
- https://doi.org/10.1016/0014-5793(91)80565-k
Abstract
The high-affinity nickel transporter of Alcaligenes eutrophus H16 is encoded by gene hoxN, which maps within the hydrogenase gene cluster of megaplasmid pHG1. A tripartite gene fusion was constructed, consisting of (i) the Escherichia coli lacZ gene for β-galactoxidase, (ii) a segment encoding an endoproteolytically cleavable peptide, and (iii) the A. eutrophus gene hoxN. An E. coli strain harboring this construct (plasmid pCH307) efficiently produced the corresponding triprotein upon induction. A broad-host-range derivative of pCH307 was shown to complement an A. eutrophus HoxN− mutant.Keywords
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