Pantetheinase activity of membrane‐bound Vanin‐1: lack of free cysteamine in tissues of Vanin‐1 deficient mice
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Open Access
- 20 October 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 483 (2-3), 149-154
- https://doi.org/10.1016/s0014-5793(00)02110-4
Abstract
Pantetheinase (EC 3.5.1.‐) is an ubiquitous enzyme which in vitro has been shown to recycle pantothenic acid (vitamin B5) and to produce cysteamine, a potent anti‐oxidant. We show that the Vanin‐1 gene encodes pantetheinase widely expressed in mouse tissues: (1) a pantetheinase activity is specifically expressed by Vanin‐1 transfectants and is immunodepleted by specific antibodies; (2) Vanin‐1 is a GPI‐anchored pantetheinase, and consequently an ectoenzyme; (3) Vanin‐1 null mice are deficient in membrane‐bound pantetheinase activity in kidney and liver; (4) in these organs, a major metabolic consequence is the absence of detectable free cysteamine; this demonstrates that membrane‐bound pantetheinase is the main source of cysteamine in tissues under physiological conditions. Since the Vanin‐1 molecule was previously shown to be involved in the control of thymus reconstitution following sublethal irradiation in vivo, this raises the possibility that Vanin/pantetheinase might be involved in the regulation of some immune functions maybe in the context of the response to oxidative stress.Keywords
This publication has 31 references indexed in Scilit:
- Metallothionein-Null Mice Express Altered Genes during DevelopmentBiochemical and Biophysical Research Communications, 2000
- Nitrilase Catalyzes Amide Hydrolysis as Well as Nitrile HydrolysisBiochemical and Biophysical Research Communications, 1998
- Two Human Genes Related to Murine Vanin-1 Are Located on the Long Arm of Human Chromosome 6Genomics, 1998
- Thermal resistance of pantetheine hydrolaseBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1996
- Conformational changes in pantetheine hydrolase as a function of guanidinium chloride concentrationJournal of Protein Chemistry, 1995
- A Kinetic Study on Pantetheinase Inhibition by DisulfidesJBIC Journal of Biological Inorganic Chemistry, 1994
- A Kinetic Study on Pantetheinase Inhibition by DisulfidesJBIC Journal of Biological Inorganic Chemistry, 1994
- Enzymatic synthesis ofBiochimica et Biophysica Acta (BBA) - General Subjects, 1992
- Mouse thymic epithelial cell lines interact with and select a CD3lowCD4+ CD8+ thymocyte subset through an LFA-1-dependent adhesion -de-adhesion mechanismInternational Immunology, 1990
- Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genesNature, 1988