Single-Cell Fluorescence Imaging Using Metal Plasmon-Coupled Probe 2: Single-Molecule Counting on Lifetime Image
- 15 March 2008
- journal article
- letter
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 8 (4), 1179-1186
- https://doi.org/10.1021/nl080093z
Abstract
Multiple Alexa Fluor 647-conjugated concanavalin A (conA) molecules were covalently bound to a single 20 nm silver particle to synthesize metal plasmon-coupled probes (PCPs). The fluorescence images were recorded by scanning confocal microscopy in both intensity and lifetime. The brightness of PCPs was 30-fold brighter than those of free conA and the lifetime of PCPs was shortened dramatically. PCPs were used to label T-lymphocytic (PM1) cells. The emission spots by PCPs bound on the cell surfaces were separated clearly from the cell images by autofluorescence due to the brighter signal and shorter lifetime of PCPs. The emission spots by PCPs were also scanned in three dimensions to count the distribution of bound fluorophores on the cell surfaces. The metal-associated fluorophores thus are suggested using as novel molecular imaging agents to quantify the components and describe their distributions on the cell surfaces.Keywords
This publication has 52 references indexed in Scilit:
- Single-Molecule Studies on Fluorescently Labeled Silver Particles: Effects of Particle SizeThe Journal of Physical Chemistry C, 2007
- Fluorescence Images of DNA-Bound YOYO between Coupled Silver ParticlesLangmuir, 2007
- Metal-Enhanced Single-Molecule Fluorescence on Silver Particle Monomer and Dimer: Coupling Effect between Metal ParticlesNano Letters, 2007
- Single Cell Fluorescence Imaging Using Metal Plasmon-Coupled ProbeBioconjugate Chemistry, 2007
- Enhanced Fluorescence of Cy5-Labeled Oligonucleotides Near Silver Island Films: A Distance Effect Study Using Single Molecule SpectroscopyThe Journal of Physical Chemistry B, 2006
- Distance-Dependent Emission from Dye-Labeled Oligonucleotides on Striped Au/Ag Nanowires: Effect of Secondary Structure and Hybridization EfficiencyJournal of the American Chemical Society, 2006
- Enhanced Fluorescence of Cy5-Labeled DNA Tethered to Silver Island Films: Fluorescence Images and Time-Resolved Studies Using Single-Molecule SpectroscopyAnalytical Chemistry, 2006
- Metal-Enhanced Fluoroimmunoassay on a Silver Film by Vapor DepositionThe Journal of Physical Chemistry B, 2005
- Surface-Enhanced Fluorescence of Fluorescein-Labeled Oligonucleotides Capped on Silver NanoparticlesThe Journal of Physical Chemistry B, 2005
- In vitro cytotoxity of silver: implication for clinical wound careBurns, 2004