Infectivity titration of a prototype strain of hepatitis E virus in cynomolgus monkeys
- 1 June 1994
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 43 (2), 135-142
- https://doi.org/10.1002/jmv.1890430207
Abstract
The infectivity titer of a standard stock of the SAR‐55 strain of hepatitis E virus (HEV) was determined in cynomolgus macaques (Macaca fas cicularis) and the effect of dose on the course of the infection was examined by weekly monitoring of alanine aminotransferase (ALT) and anti‐HEV levels. Antibody to HEV (anti‐HEV) was measured with ELISAs based on ORF‐2 recombinant antigens consisting of either a 55 kDa region expressed in insect cells or shorter regions expressed as fusion proteins in bacteria. The ELISA based on the 55 kDa antigen was generally more sensitive. The infectivity titer of SAR‐55 was 106 cynomolgus 50% infectious doses per gram of feces. The infectivity titer corresponded to the HEV genome titer of the inoculum as determined by reverse transcriptase‐polymerase chain reaction (RT‐PCR). Anti‐HEV IgM was detected in only a portion of the animals that had an anti‐HEV IgG response. Biochemical evidence of hepatitis was most prominent in animals that were inoculated with the higher concentrations of virus and the incubation period to seroconversion was prolonged in animals that received the lower doses. © 1994 Wiley‐Liss, Inc.†Keywords
This publication has 24 references indexed in Scilit:
- Partial sequence comparison of eight new Chinese strains of hepatitis E virus suggests the genome sequence is relatively stableJournal of Medical Virology, 1993
- Variation in Course of Hepatitis E in Experimentally Infected Cynomolgus MonkeysThe Journal of Infectious Diseases, 1993
- Infection of Owl Monkeys (Aotus trivirgatus) and Cynomolgus Monkeys (Macaca fascicularist) with Hepatitis E Virus from MexicoThe Journal of Infectious Diseases, 1992
- Serologic identification of hepatitis E virus infections in epidemic and endemic settingsJournal of Medical Virology, 1992
- Enzyme-linked immunosorbent assay for diagnosis of acute sporadic hepatitis E in Egyptian childrenThe Lancet, 1992
- Association of hepatitis E virus with an outbreak of hepatitis in Pakistan: Serologic responses and pattern of virus excretionJournal of Medical Virology, 1992
- Hepatitis C viral RNA in serum of patients with chronic non-A, non-B hepatitis: Detection by the polymerase chain reaction using multiple primer setsHepatology, 1991
- Isolation of a cDNA from the Virus Responsible for Enterically Transmitted Non-A, Non-B HepatitisScience, 1990
- OUTBREAK OF ENTERICALLY TRANSMITTED NON-A, NON-B HEPATITIS AMONG SCHOOLCHILDRENThe Lancet, 1988
- Evidence for a Virus in Non-A, Non-B Hepatitis Transmitted via the Fecal-Oral RouteIntervirology, 1982