Members of the α‐amylase inhibitors family from wheat endosperm are major allergens associated with baker's asthma
- 12 February 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 261 (1), 85-88
- https://doi.org/10.1016/0014-5793(90)80642-v
Abstract
We have identified the major antigens or IgE binding components from wheat flour. Thirty-five sera from patients with baker's asthma were used to analyze the reaction with wheat salt-soluble proteins. We found a 15 kDa SDS-PAGE band which reacted with all sera tested. Purified members of the α-amylase inhibitor family, which are the main components of the 15 kDa band, were recognized by specific IgE when tested with a pool of reactive sera. Immunodetection after two-dimensional electrophoretic fractionation of crude inhibitor preparations from wheat endosperms also detected several inhibitor subunits as major low-molecular-weight allergens.Keywords
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