Determination of Ketoconazole in Pharmaceutical Preparations by Ultraviolet Spectrophotometry and High Performance Liquid Chromatography

Abstract

The aim of this research was to study and to standardize an ultraviolet spectrophotometric (UVS) and a high performance liquid chromatographic (HPLC) method for the determination of ketoconazole in commercially available pharmaceutical preparations (tablets and creams). The UVS method was both standardized at 222 nm and 269 nm in 0.01M HCl. Beer's law was obeyed in a range of concentration from 4.0 to 13.0 μg/mL at 222 nm and from 100.0 to 280.0 μg/mL at 269 nm. In the HPLC determination were used a Merck LiChrospher^R 100 RP-18 (5 μm) in LiChroCART^R (125–4) column, a mobile phase consisting of diisopropylamine-methanol 1:500/ammonium acetate 1:200 (8:2) and UV detection at 225 nm. There was a correspondence in areas “versus” concentration in a range from 2.0 to 18.0 μg/mL.