HELP (High Efficiency Liquid Phase) new oligonucleotide synthesis on soluble polymeric support
- 1 January 1990
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (11), 3155-3159
- https://doi.org/10.1093/nar/18.11.3155
Abstract
A simple, rapid and high-yielding method for the synthesis of oligonucleotides by the phosphotriesters approach is described. The use of polyethylene glycol (PEG) as soluble polymeric support preserves some convenient features of the solid-phase synthesis with new interesting advantages. Short oligonucleotides in hundred milligrams scale can be obtained from few grams of functionalized PEG.Keywords
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