Targeted deletion of the osteoclast protein‐tyrosine phosphatase (PTP‐oc) promoter prevents RANKL‐mediated osteoclastic differentiation of RAW264.7 cells
- 4 May 2007
- journal article
- Published by Wiley in FEBS Letters
- Vol. 581 (13), 2503-2508
- https://doi.org/10.1016/j.febslet.2007.04.063
Abstract
An osteoclastic protein-tyrosine phosphatase, PTP-oc, shares the same gene with a renal PTP, Glepp1. This study demonstrated that targeted deletion of PTP-oc promoter by homologous recombination in RAW264.7 cells completely abolished PTP-oc expression without affecting Glepp1 expression. This strategy to inhibit PTP-oc function has three advantages over commonly used gene knock down strategies (e.g., small interference RNA). This strategy: (1) yielded cells completely devoid of PTP-oc, (2) had no off-target gene silencing effects, and (3) did not affect Glepp1 expression. The inability of PTP-oc-deficient RAW264.7 cells to undergo RANKL-mediated osteoclastic differentiation confirmed a regulatory role for PTP-oc in RANKL-mediated osteoclast differentiationKeywords
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