Microbial Degradation of Polyhydroxyalkanoates
Top Cited Papers
- 1 October 2002
- journal article
- review article
- Published by Annual Reviews in Annual Review of Microbiology
- Vol. 56 (1), 403-432
- https://doi.org/10.1146/annurev.micro.56.012302.160838
Abstract
▪ Abstract Polyesters such as poly(3-hydroxybutyrate) (PHB) or other polyhydroxyalkanoates (PHA) have attracted commercial and academic interest as new biodegradable materials. The ability to degrade PHA is widely distributed among bacteria and fungi and depends on the secretion of specific extracellular PHA depolymerases (e-PHA depolymerases), which are carboxyesterases (EC 3.1.1.75 and EC 3.1.1.76), and on the physical state of the polymer (amorphous or crystalline). This contribution provides a summary of the biochemical and molecular biological characteristics of e-PHA depolymerases and focuses on the intracellular mobilization of storage PHA by intracellular PHA depolymerases (i-PHA depolymerases) of PHA-accumulating bacteria. The importance of different assay systems for PHA depolymerase activity is also discussed.Keywords
This publication has 89 references indexed in Scilit:
- Transfer of [Pseudomonas] lemoignei, a gram-negative rod with restricted catabolic capacity, to Paucimonas gen. nov. with one species, Paucimonas lemoignei comb. nov.International Journal of Systematic and Evolutionary Microbiology, 2001
- Poly(3-hydroxybutyrate) depolymerases bind to their substrate by a C-terminal located substrate binding siteFEMS Microbiology Letters, 1996
- Intracellular poly(3-hydroxybutyrate) depolymerase inAlcaligenes eutrophusCanadian Journal of Microbiology, 1995
- Pseudomonas lemoignei has five poly(hydroxyalkanoic acid) (PHA) depolymerase genes: A comparative study of bacterial and eukaryotic PHA depolymerasesJournal of Polymers and the Environment, 1994
- A method for the isolation of microorganisms producing extracellular long-side-chain poly (β-hydroxyalkanoate) depolymeraseJournal of Polymers and the Environment, 1994
- Intracellular degradation of poly(3-hydroxybutyrate) granules ofZoogloea ramigeraI-16-MFEMS Microbiology Letters, 1992
- Effect of limited tryptic modification of a bacterial poly(3-hydroxybutyrate) depolymerase on its catalytic activityBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1988
- Purification and properties of extracellular poly(3-hydroxybutyrate) depolymerases from Pseudomonas lemoigneiBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
- Metabolism of poly(β-hydroxybutyrate). I. Purification, composition, and properties of native poly(β-hydroxybutyrate) granules from Bacillus megateriumBiochemistry, 1968
- Poly- -hyroxybutyrate Metabolism in Washed Suspensions of Bacillus cereus and Bacillus megateriumJournal of General Microbiology, 1958